Description
CatalogueNumber	AB1585
Replaces	AB145
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-DopamineβHydroxylaseAntibody
AlternateNames	DBH Dopaminebeta-monooxygenase

ProductInformation
Format	Serum
Control	Brain(Striatum/SubstantiaNigra)
Presentation	RabbitSerum.Lyophilized,nopreservatives.Reconstitutewith50μLofsteriledistilledwater.Centrifugetoremoveanyinsolublematerial.

StorageandShippingInformation
StorageConditions	Maintainlyophilizedmaterialat-20to-70°Cforupto12monthsafterdateofreceipt.Afterreconstitutionmaintainat-20to-70°Cinundilutedaliquotsforuptosixmonths.Avoidrepeatedfreeze/thawcycles.Glycerolcanbeadded1:1foradditionalstABIlity.

Applications
Application	DetectDopamineβHydroxylaseusingthisAnti-DopamineβHydroxylaseAntibodyvalidatedforuseinIH,FUNC,RIA&WB.
KeyApplications	Immunohistochemistry AffectsFunction RADIoimmunoassay WesternBlotting
ApplicationNotes	Immunohistochemistry:1:2,000onrabbitandguineapigusinganABCdetectionmethod. Westernblot RIA Inhibitorystudiesinvivoandinvitro. Optimalworkingdilutionsmustbedeterminedbytheenduser.

BIOLOGicalInformation
Immunogen	Nativeenzymefrombovineadrenalglands.
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Rabbit
Specificity	RecognizesDopaminebetahydroxylase.AB1585reactswithasinglebandonWesternblotsofbovineadrenalhomogenatesandstainsonlycellsknowntocontainDBH,suchassympatheticneurons,adrenalmedullarycellsandcentraladrenergicneurons.Stainingisabolishedbypreincubationwithenzyme.
SpeciesReactivity	Bovine GuineaPig Human Rat Rabbit Feline
AntibodyType	PolyclonalAntibody
EntrezGeneNumber	NM_000787.3
EntrezGeneSummary	TheproteinencodedbythisgeneisanoxidoreductasebelongingtothecoppertypeII,ascorbate-dependentmonooxygenasefamily.Itispresentinthesynapticvesiclesofpostganglionicsympatheticneuronsandconvertsdopaminetonorepinephrine.Itexistsinbothsolubleandmembrane-boundforms,dependingontheabsenceorpresence,respectively,ofasignalpeptide.
GeneSymbol	DBH DBM EC1.14.17.1[Contains:Solubledopaminebeta-hydroxylase].
PurificationMethod	Unpurified
UniProtNumber	P09172
UniProtSummary	FUNCTION:SwissProt:P09172#Conversionofdopaminetonoradrenaline. COFACTOR:Binds1PQQpersubunit.&Binds2copperionspersubunit. SIZE:617aminoacids;69065Da SUBUNIT:Homotetramercomposedoftwonon-covalentlybounddisulfide-linkeddimers. SUBCELLULARLOCATION:Solubledopaminebeta-hydroxylase:Cytoplasmicvesicle,secretoryvesiclelumen.Cytoplasmicvesicle,secretoryvesicle,chromaffingranulelumen.&Cytoplasmicvesicle,secretoryvesiclemembrane;Single-passtypeIImembraneprotein.Cytoplasmicvesicle,secretoryvesicle,chromaffingranulemembrane;Single-passtypeIImembraneprotein(Potential). DISEASE:SwissProt:P09172#DefectsinDBHarethecauseofDBHdeficiency[MIM:223360];alsocallednorepinephrinedeficiencyornoradrenalinedeficiency.Thisdisorderischaracterizedbyprofounddeficitsinautonomicandcardiovascularfunction,butapparentlyonlysubtlesigns,ifany,ofcentralnervoussystemdysfunction. SIMILARITY:SwissProt:P09172##BelongstothecoppertypeIIascorbate-dependentmonooxygenasefamily.&Contains1DOMONdomain.
MolecularWeight	69kDa

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

Millipore 提供三种不同的 Immobilon PVDF 膜，是针对不同的蛋白质印迹应用的理想选择。 现在我们还 提供采用按规格裁切的印迹方片膜和两层过滤纸组成的印迹三明治。   与硝酸纤维素膜相比，Immobilon 膜具有多种优点。 它们不易破裂或卷 曲，便于裁剪且在切割过程中不会碎裂。 它们还有低背景、广泛溶剂相容性以及优异的着色能力。 此外，它们可以重复检测。 Immobilon-P 膜（沃比森-供应） ·         0.45μm 孔径 ·         建议用于大多数免疫印迹，特别在蛋 白质大于 20kDa 时 ·         Millipore 的快速免疫检测实验方法不需要膜封闭并缩短洗涤时间，将检测时间至少减少了两个小时，而不致降低灵敏 度或特异性 用于高通量实验室的印迹三明治 ·         当您需要处理多个印迹时，印迹三明治是一种便利省时的选择。 我们的印迹三明 治包含多片 Immobilon-P 膜，其中插入了按规格裁切的多张层析级印迹纸。 转印滤纸 ·         按照大多数免疫印迹尺寸进行裁切的层析级转印滤纸。 Immobilon-PSQ 膜（沃比森-供应） ·         0.2μm 孔径和大的内表面面积 ·         较其它膜具有更高的 蛋白质吸附率和测序得率 ·         建议用于小于 20kDa 的印迹蛋白质 ·         防止小分子量的蛋白质的渗漏 Immobilon-FL 膜（沃比森-供应） ·        针对荧光应用进行优化的转印膜 ·         极低的背景提高了所 有荧光检测实验的灵敏度 ·         与所有常用的荧光探针（所有激发和发射波长）兼容 ·         是多态性检测和化学荧光应用的理想选择