Description
CatalogueNumber	AB5334P
Replaces	04-1053
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-SynucleinαAntibody

ProductInformation
Format	AffinityPurified
Presentation	Affinitypurifiedimmunoglobulin.Lyophilized.Reconstitutewith50μLofsteriledistilledwater.Centrifugetoremoveanyresidue.Glycerol(1:1)canbeaddedforadditionalstABIlity.

StorageandShippingInformation
StorageConditions	Maintainlyophilizedmaterialat-70°C(dry)forupto12months.Afterreconstitutionmaintainat-20°Cinundilutedaliquotsforupto6months.Avoidrepeatedfreeze/thawcycles.

Applications
Application	DetectSynucleinusingthisAnti-SynucleinAntibody,αvalidatedforuseinWB,IH(P).
KeyApplications	WesternBlotting Immunohistochemistry(Paraffin)
ApplicationNotes	Immunohistochemistry:0.5-1μg/mLonfrozenorparaffinsections. Westernblot:0.5-1μg/mL Optimalworkingdilutionsmustbedeterminedbytheenduser.

BIOLOGicalInformation
Immunogen	Syntheticpeptidecorrespondingaminoacids116-131ofhumanalphasynuclein.
Host	Sheep
Specificity	Recognizesalphasynuclein.
SpeciesReactivity	Human Rat
AntibodyType	PolyclonalAntibody
EntrezGeneNumber	NM_000345.2 NM_007308.1
EntrezGeneSummary	Alpha-synucleinisamemberofthesynucleinfamily,whichalsoincludesbeta-andgamma-synuclein.Synucleinsareabundantlyexpressedinthebrainandalpha-andbeta-synucleininhibitphospholipaseD2selectively.SNCAmayservetointegratepresynapticsignalingandmembranetrafficking.DefectsinSNCAhavebeenimplicatedinthepathogenesisofParkinsondisease.SNCApeptidesareamajorcomponentofamyloidplaquesinthebrainsofpatientswithAlzheimer"sdisease.TwoalternativelysplicedtranscriptsofSNCAhavebeenidentified.Additionalsplicingmaybepresentbutthefull-lengthnatureofthesevariantshasnotbeendetermined.
GeneSymbol	SNCA PD1 alpha-synuclein NACP PARK4 MGC110988 PARK1 Alpha-synuclein
UniProtNumber	P37840
UniProtSummary	FUNCTION:SwissProt:P37840#Maybeinvolvedintheregulationofdopaminereleaseandtransport.Solubleprotein,normallylocalizedprimarilyatthepresynapticregionofaxons,whichcanformfilamentousaggregatesthatarethemajornonamyloidcomponentofintracellularinclusionsinseveralneurodegenerativediseases(synucleinopathies).Inducesfibrillizationofmicrotubule-associatedproteintau.Reducesneuronalresponsivenesstovariousapoptoticstimuli,leADIngtoadecreasedcaspase3activation. SIZE:140aminoacids;14460Da SUBUNIT:Solublemonomerwhichcanformfilamentousaggregates.InteractswithUCHL1(Bysimilarity).InteractswithphospholipaseDandhistones. SUBCELLULARLOCATION:Cytoplasm.Membrane.Nucleus.Note=Membrane-boundindopaminergicneurons.Alsofoundinthenucleus. TISSUESPECIFICITY:Expressedprincipallyinbrainbutisalsoexpressedinlowconcentrationsinalltissuesexaminedexceptinliver.Concentratedinpresynapticnerveterminals. DOMAIN:SwissProt:P37840TheNACdomainisinvolvedinthefibrilformation.Themiddleregionformsthecoreofthefilaments.TheC-terminusmayregulateaggregationanddeterminethediameterofthefilaments. PTM:Phosphorylated,predominantlyonserineresidues.PhosphorylationbyCK1appearstooccuronresiduesdistinctfromtheresiduephosphorylatedbyotherkinases.PhosphorylationofSer-129isselectiveandextensiveinsynucleinopathylesions.Invitro,phosphorylationatSer-129promotedinsolublefibrilformation.PhosphorylatedonTyr-125byaPTK2B-dependentpathwayuponosmoticstress.&Hallmarklesionsofneurodegenerativesynucleinopathiescontainalpha-synucleinthatismodifiedbynitrationoftyrosineresiduesandpossIBLybydityrosinecross-linkingtogeneratedstableoligomers.&Ubiquitinated.Thepredominantconjugateisthediubiquitinatedform(Bysimilarity). DISEASE:SwissProt:P37840#DefectsinSNCAareacauseofautosomaldominantParkinsondisease1(PARK1)[MIM:168601,168600].Parkinsondisease(PD)isacomplex,multifactorialdisorderthattypicallymanifestsaftertheageof50years,althoughearly-onsetcases(before50years)areknown.PDgenerallyarisesasasporadicconditionbutisoccasionallyinheritedasasimplemendeliantrait.AlthoughsporadicandfamilialPDareverysimilar,inheritedformsofthediseaseusuallybeginatearlieragesandareassociatedwithatypicalclinicalfeatures.PDischaracterizedbybradykinesia,restingtremor,muscularrigidityandposturalinstability,aswellasbyaclinicallysignificantresponsetotreatmentwithlevodopa.ThepathologyinvolvesthelossofdopaminergicneuronsinthesubstantianigraandthepresenceofLewybodies(intraneuronalaccumulationsofaggregatedproteins),insurvivingneuronsinvariousareasofthebrain.&DefectsinSNCAarethecauseofParkinsondisease4(PARK4)[MIM:605543,168600].&DefectsinSNCAarethecauseofLewybodydementia(DLB)[MIM:127750].DLBisaneurodegenerativedisorderclinicallycharacterizedbydementiaandparkinsonism,oftenwithfluctuatingcognitivefunction,visualhallucinations,falls,syncopalepisodes,andsensitivitytoneurolepticmedication.PresenceofLewybodiesaretheonlyessentialpathologicfeatures.&DepositionoffibrillaramyloidproteinsintraneuronallyasneurofibrillarytanglesischaracteristicofAlzheimerdisease(AD).SNCAisaminorproteinfoundwithinthesedeposits,butamajornonamyloidcomponent.&Brainironaccumulationtype1(NBIA1,alsocalledHallervorden-Spatzsyndrome),arareneuroaxonaldystrophy,ishistologicallycharacterizedbyaxonalspheroids,irondeposition,Lewybody(LB)-likeintraneuronalinclusions,glialinclusionsandneurofibrillarytangles.SNCAisfoundinLB-likeinclusions,glialinclusionsandspheroids. SIMILARITY:Belongstothesynucleinfamily.

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

Millipore 提供三种不同的 Immobilon PVDF 膜，是针对不同的蛋白质印迹应用的理想选择。 现在我们还 提供采用按规格裁切的印迹方片膜和两层过滤纸组成的印迹三明治。   与硝酸纤维素膜相比，Immobilon 膜具有多种优点。 它们不易破裂或卷 曲，便于裁剪且在切割过程中不会碎裂。 它们还有低背景、广泛溶剂相容性以及优异的着色能力。 此外，它们可以重复检测。 Immobilon-P 膜（沃比森-供应） ·         0.45μm 孔径 ·         建议用于大多数免疫印迹，特别在蛋 白质大于 20kDa 时 ·         Millipore 的快速免疫检测实验方法不需要膜封闭并缩短洗涤时间，将检测时间至少减少了两个小时，而不致降低灵敏 度或特异性 用于高通量实验室的印迹三明治 ·         当您需要处理多个印迹时，印迹三明治是一种便利省时的选择。 我们的印迹三明 治包含多片 Immobilon-P 膜，其中插入了按规格裁切的多张层析级印迹纸。 转印滤纸 ·         按照大多数免疫印迹尺寸进行裁切的层析级转印滤纸。 Immobilon-PSQ 膜（沃比森-供应） ·         0.2μm 孔径和大的内表面面积 ·         较其它膜具有更高的 蛋白质吸附率和测序得率 ·         建议用于小于 20kDa 的印迹蛋白质 ·         防止小分子量的蛋白质的渗漏 Immobilon-FL 膜（沃比森-供应） ·        针对荧光应用进行优化的转印膜 ·         极低的背景提高了所 有荧光检测实验的灵敏度 ·         与所有常用的荧光探针（所有激发和发射波长）兼容 ·         是多态性检测和化学荧光应用的理想选择