Description
CatalogueNumber	04-958
Replaces	04-1002
BrandFamily	Upstate
TradeName	Upstate
Description	Anti-BetaCateninAntibody,clone7F7.2
AlternateNames	BetaCatenin Catenin(cadherin-associatedprotein),beta1
BackgroundInformation	Cateninsareproteinsfoundincomplexeswithcadherincelladhesionmoleculesofanimalcells.Thefirsttwocateninsthatwereidentifiedbecameknownasα-cateninandβ-catenin.α-catenincanbindtoβ-cateninandcanalsobindactin.β-cateninbindsthecytoplasmicdomainofsomecadherins.Whenβ-cateninwassequenceditwasfoundtobeamemberofthearmADIllofamilyofproteins.Theseproteinshavemultiplecopiesoftheso-calledarmadillorepeatdomainwhichisspecializedforprotein-proteinbinding.Anincreaseinβ-cateninproductionhasbeennotedinthosepeoplewhohaveBasalCellCarcinomaandleadstotheincreaseinproliferationofrelatedtumors.Whenβ-cateninisnotassociatedwithcadherinsandα-catenin,itcaninteractwithotherproteinssuchasCateninBetaInteractingProtein1(ICAT)andadenomatosispolyposiscoli(APC).Recentevidencesuggeststhatβ-cateninplaysanimportantroleinvariousaspectsofliverBIOLOGyincludingliverdevelopment(bothembryonicandpostnatal),liverregenerationfollowingpartialhepatectomy,HGF-inducedhepatpomegaly,liverzonation,andpathogenesisoflivercancer.

ProductInformation
Format	Purified
Control	HEK293celllysate,PC12celllysate,NIH/3T3celllysate
Presentation	PurifiedmousemonoclonalIgG1κinbuffercontaining0.1MTris-Glycine,pH7.4,150mMNaClwith0.05%sodiumazide.

StorageandShippingInformation
StorageConditions	Stablefor1yearat2-8°Cfromdateofreceipt.

Applications
Application	ThisAnti-BetaCateninAntibody,clone7F7.2isvalidatedforuseinWB,ICforthedetectionofBetaCatenin.
KeyApplications	WesternBlotting Immunocytochemistry
ApplicationNotes	WesternBlotAnalysis:0.5-2µg/mLofthislotdetectedbetacatenininHEK293celllysate.

BiologicalInformation
Immunogen	Fulllength,GST-taggedrecombinantbetacatenin.
Clone	7F7.2
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Mouse
Specificity	Thisantibodyrecognizesbetacatenin.
Isotype	IgG1κ
SpeciesReactivity	Human Mouse Rat
AntibodyType	MonoclonalAntibody
EntrezGeneNumber	NP_001895
EntrezGeneSummary	Beta-cateninisanadherensjunctionprotein.Adherensjunctions(AJs;alsocalledthezonulaadherens)arecriticalfortheestablishmentandmaintenanceofepitheliallayers,suchasthoseliningorgansurfaces.AJsmediateadhesionbetweencells,communicateasignalthatneighboringcellsarepresent,andanchortheactinCytoskeleton.Inservingtheseroles,AJsregulatenormalcellgrowthandbehavior.Atseveralstagesofembryogenesis,woundhealing,andtumorcellmetastasis,cellsformandleaveepithelia.Thisprocess,whichinvolvesthedisruptionandreestablishmentofepithelialcell-cellcontacts,mayberegulatedbythedisassemblyandassemblyofAJs.AJsmayalsofunctioninthetransmissionofthe"contactinhibition"signal,whichinstructscellstostopdividingonceanepithelialsheetiscomplete.[suppliedbyOMIM]
GeneSymbol	CTNNB CTNNB1 FLJ25606 FLJ37923 DKFZp686D02253
PurificationMethod	ProteinGPurified
UniProtNumber	P35222
UniProtSummary	FUNCTION:InvolvedintheregulationofcelladhesionandinsignaltransductionthroughtheWntpathway. SUBUNIT:Twoseparatepoolsarefoundinthecytoplasm:oneisPSEN1/cadherin/catenincomplexwhichanchorstotheactincytoskeleton.TheotherpoolispartofalargecomplexcontainingAXIN1,AXIN2,APC,CSNK1A1andGSK3BthatpromotesphosphorylationonN-terminalSerandThrresiduesandubiquitinationofCTNNB1viaBTRCanditssubsequentdegradationbytheproteasome.Wnt-dependentactivationofDVLantagonizestheactionofGSK3B.WhenGSK3Bactivityisinhibitedthecomplexdissociates,CTNNB1isdephosphorylatedandisnolongertargetedfordestruction.ThestABIlizedproteintranslocatestothenucleus,whereitbindsTCF/LEF-1familymembers,TBP,BCL9andpossIBLyalsoRUVBL1andDUPLIN.BindsCTNNBIPandEP300.CTNNB1formsaternarycomplexwithLEF1andEP300thatisdisruptedbyCTNNBIP1binding(Bysimilarity).InteractswiththePDZdomainofTAX1BP3;thisinteractioninhibitsthetranscriptionalactivityofCTNNB1(Bysimilarity).InteractswithAJAP1,BAIAP1,CARM1,CTNNA3,CXADRandPCDH11Y.BindsSLC9A3R1.InteractswithGLIS2andMUC1.InteractswithSLC30A9.InteractswithXIRP1(Bysimilarity). SUBCELLULARLOCATION:Cytoplasm.Nucleus.Cytoplasm,cytoskeleton.Note=Cytoplasmicwhenitisunstabilized(highlevelofphosphorylation)orboundtoCDH1.Translocatestothenucleuswhenitisstabilized(lowlevelofphosphorylation).InteractionwithGLIS2andMUC1promotesnucleartranslocation. TISSUESPECIFICITY:Expressedinseveralhairfolliclecelltypes:basalandperipheralmatrixcells,andcellsoftheouterandinnerrootsheats.Expressedincolon.
MolecularWeight	92kDa

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

Millipore 提供三种不同的 Immobilon PVDF 膜，是针对不同的蛋白质印迹应用的理想选择。 现在我们还 提供采用按规格裁切的印迹方片膜和两层过滤纸组成的印迹三明治。   与硝酸纤维素膜相比，Immobilon 膜具有多种优点。 它们不易破裂或卷 曲，便于裁剪且在切割过程中不会碎裂。 它们还有低背景、广泛溶剂相容性以及优异的着色能力。 此外，它们可以重复检测。 Immobilon-P 膜（沃比森-供应） ·         0.45μm 孔径 ·         建议用于大多数免疫印迹，特别在蛋 白质大于 20kDa 时 ·         Millipore 的快速免疫检测实验方法不需要膜封闭并缩短洗涤时间，将检测时间至少减少了两个小时，而不致降低灵敏 度或特异性 用于高通量实验室的印迹三明治 ·         当您需要处理多个印迹时，印迹三明治是一种便利省时的选择。 我们的印迹三明 治包含多片 Immobilon-P 膜，其中插入了按规格裁切的多张层析级印迹纸。 转印滤纸 ·         按照大多数免疫印迹尺寸进行裁切的层析级转印滤纸。 Immobilon-PSQ 膜（沃比森-供应） ·         0.2μm 孔径和大的内表面面积 ·         较其它膜具有更高的 蛋白质吸附率和测序得率 ·         建议用于小于 20kDa 的印迹蛋白质 ·         防止小分子量的蛋白质的渗漏 Immobilon-FL 膜（沃比森-供应） ·        针对荧光应用进行优化的转印膜 ·         极低的背景提高了所 有荧光检测实验的灵敏度 ·         与所有常用的荧光探针（所有激发和发射波长）兼容 ·         是多态性检测和化学荧光应用的理想选择