Description
CatalogueNumber	AB5622
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-Microtubule-AssociatedProtein2(MAP2)Antibody
AlternateNames	microtubule-associatedprotein2
BackgroundInformation	Themicrotubule-associatedprotein2(MAP2)isanabundantneuronalcytoskeletalproteinthatbindstotubulinandstABIlizesmicrotubules.(HerzogandWeber,1978).MAP2isessentialforthedevelopmentandmaitenanceofneuronalmorphology(Matus,1991).InneuronsMAP2occursasthreeprimaryisoforms,thehighmolecularweightMAP2a,MAP2b,andthelowmolecularweightMAP2c,thatresultfromalternativesplicingoftheMAP2gene(Chungetal.,1996).Thelowmolecularweightisoform,MAP2c,isexpressedindevelopingbrainandisdown-regulatedduringbrainmaturation,whereasthehighmolecularweightMAP2bisexpressedinbothdevelopingandadultbrain.TheMAP2aappearsonlyafterbrainmaturation(Tucker,1990).Alltheseformsbindtomicrotubulesthroughadomainnearthecarboxylterminusthatcontainseitherthreeorfoursimilarrepeatsofa31-amino-acidmotif(Lewisetal.,1988).MAP2togetherwithMAP4andtauproteinsbelongtothefamilyofThermostableproteinsassociatedwithmicrotubules.

ProductInformation
Format	Purified
Control	HumanbraintissueorhumanglioblastomaT98Gcells
Presentation	PurifiedrabbitpolyclonalinbuffercontainingPBSwith0.1%sodiumazideasapreservative.

StorageandShippingInformation
StorageConditions	Maintainat-20ºCinundilutedaliquotsforupto6monthsafterdateofreceipt.Avoidrepeatedfreeze/thawcycles.

Applications
Application	DetectMicrotubule-AssociatedProtein2(MAP2)usingthisAnti-Microtubule-AssociatedProtein2(MAP2)AntibodyvalidatedforuseinELISA,IC,IH,IH(P)&WBwithmorethan55productcitations.
KeyApplications	ELISA Immunocytochemistry Immunohistochemistry Immunohistochemistry(Paraffin) WesternBlotting
ApplicationNotes	Immunocytochemistry: 1:1,000dilutionofapreviouslotwasusedonprimaryneurons. Immunohistochemistry: 1:1,000dilutionon4%paraformaldehyde/PBSfixedtissuesections. Immunoblot: 1:2,000dilutionwasusedonapreviouslot.RecognizesMAP2A(280-300kDa,andMAP2B(270-280KDa),andtoalesserextentMAP2C(70kDa)andMAP2D(70-75kDa). ELISA: greaterthanorequalto1:2,000dilutionofapreviouslotwasused. Optimalworkingdilutionsmustbedeterminedbytheenduser.

BIOLOGicalInformation
Immunogen	PurifiedMicrotubule-associatedproteinfromratbrain.
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Rabbit
Specificity	SpecificforMicrotubule-associatedprotein2(MAP2).TheantibodyrecognizesallMAP2isoforms(MAP2A,MAP2B,MAP2CandMAP2D).ItshowsgreatestimmunoreactivitywiththeHMW-MAP2(MAP2AandMAP2B).
SpeciesReactivity	Human Mouse Rat
SpeciesReactivityNote	Human,mouseandrat.Reactivitywithotherspecieshasnotbeendetermined.
AntibodyType	PolyclonalAntibody
EntrezGeneNumber	NM_001039538.1
EntrezGeneSummary	Thisgeneencodesaproteinthatbelongstothemicrotubule-associatedproteinfamily.Theproteinsofthisfamilyarethoughttobeinvolvedinmicrotubuleassembly,whichisanessentialstepinneurogenesis.Theproductsofsimilargenesinratandmouseareneuron-specificcytoskeletalproteinsthatareenrichedindentrites,implicatingaroleindeterminingandstabilizingdentriticshapeduringneurondevelopment.Anumberofalternativelysplicedvariantsencodingdistinctisformshavebeendescribed.
GeneSymbol	MAP2 MAP2B MAP-2 MAP2C DKFZp686I2148 MAP2A
PurificationMethod	AmmoniumSulfatePrecipitation
UniProtNumber	P11137
UniProtSummary	FUNCTION:SwissProt:P11137#TheexactfunctionofMAP2isunknownbutMAPsmaystabilizethemicrotubulesagainstdepolymerization.Theyalsoseemtohaveastiffeningeffectonmicrotubules. SIZE:1827aminoacids;199539Da PTM:MAP2A/cisphosphorylated.PhosphorylateduponDNAdamage,probablybyATMorATR. SIMILARITY:SwissProt:P11137##Contains3Tau/MAPrepeats.
MolecularWeight	Approx.280kDadoublet(MAP2aandMAP2b)andapprox.70kDadoublet(MAP2c)onSDS-PAGE.

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

Millipore 提供三种不同的 Immobilon PVDF 膜，是针对不同的蛋白质印迹应用的理想选择。 现在我们还 提供采用按规格裁切的印迹方片膜和两层过滤纸组成的印迹三明治。   与硝酸纤维素膜相比，Immobilon 膜具有多种优点。 它们不易破裂或卷 曲，便于裁剪且在切割过程中不会碎裂。 它们还有低背景、广泛溶剂相容性以及优异的着色能力。 此外，它们可以重复检测。 Immobilon-P 膜（沃比森-供应） ·         0.45μm 孔径 ·         建议用于大多数免疫印迹，特别在蛋 白质大于 20kDa 时 ·         Millipore 的快速免疫检测实验方法不需要膜封闭并缩短洗涤时间，将检测时间至少减少了两个小时，而不致降低灵敏 度或特异性 用于高通量实验室的印迹三明治 ·         当您需要处理多个印迹时，印迹三明治是一种便利省时的选择。 我们的印迹三明 治包含多片 Immobilon-P 膜，其中插入了按规格裁切的多张层析级印迹纸。 转印滤纸 ·         按照大多数免疫印迹尺寸进行裁切的层析级转印滤纸。 Immobilon-PSQ 膜（沃比森-供应） ·         0.2μm 孔径和大的内表面面积 ·         较其它膜具有更高的 蛋白质吸附率和测序得率 ·         建议用于小于 20kDa 的印迹蛋白质 ·         防止小分子量的蛋白质的渗漏 Immobilon-FL 膜（沃比森-供应） ·        针对荧光应用进行优化的转印膜 ·         极低的背景提高了所 有荧光检测实验的灵敏度 ·         与所有常用的荧光探针（所有激发和发射波长）兼容 ·         是多态性检测和化学荧光应用的理想选择