Description
CatalogueNumber	ECM200
TradeName	QCM Chemicon
Description	QCM3µmEndothelialCellMigrationAssayFibronectin,Colorimetric
Overview	Alsoavailable:CellComb™ScratchAssay!Getbiochemicaldatafromascratchassay!ClickHere Introduction Angiogenesisisafundamentalprocessinvolvingthegrowthofnewbloodvesselsfrompre-existingvessels.Itisimportantindevelopmentandwoundhealing,aswellaspathologicdiseasessuchasdiabeticretinopathyandcancer.Duringangiogenesis,endothelialcellsneedtomoveoutofexistingvessels,migrateintonewareas,proliferateandassembleintonewcapillaries.Themigrationofendothelialcellsisregulatedbymanyangiogenicfactorsandanti-angiogenicfactors.Itiscriticalforresearcherstounderstandthemechanismsofendothelialcellmigration. Millipore"s3μmQCM™EndothelialCellMigrationAssay–Fibronectin,ColorimetricprovidesaquickandefficientsystemtostudytheABIlityofcompoundstoinduceorinhibitendothelialcellmigration.Thisassayalsoallowsscreeningofpharmacologicalagents,evaluationofintegrinsorotheradhesionreceptorsresponsIBLeforendothelialcellmigration,analysisofgenefunctionintransfectedcells,anddeterminationofECMproteininvolvementincellmovement. Thisversatileassaypermitscountingofindividualmigratorycells,and,moreimportantly,allowsquantitativeanalysisbyopticaldensity(OD)usingastandardmicroplatereader.Thisconvenientassayallowslargescalescreeningandquantitativecomparisonofmultiplesamplesandincludesindividualmigrationcontrolsforeachsample.
MaterialsRequiredbutNotDelivered	1.PrecisionPipettes:sufficientforaliquotingcells. 2.Harvestingbuffer:EDTAortrypsinbasedcelldetachmentbuffer,orothercelldetachmentformulationsasoptimizedbyindividualinvestigators.Millipore’sready-to-usenon-mammaliandetachmentsolution,Accutase™(Cat.No.SCR005)canalsobeused. 3.Endothelialcells(forexample:HUVECcells). 4.Endotheliumcellculturemediumappropriateforsubjectcells,suchasEGM-2(Endothelialcellgrowthmedia-2) 5.QuenchingMedium:serum-freemedium,suchasDMEM,MEMetccontaining5%BSA.Mustcontaindivalentcations(Mg2+,Ca2+)sufficientforquenchingEDTAinharvestingbuffer. 6.SterilePBSorHBSStowashcells. 7.Distilledwater 8.(Optional)Chemoattractantorpharmacologicalagentaddedtoculturemedium. 9.Lowspeedcentrifugeandtubesforcellharvesting. 10.CO2incubatorappropriateforsubjectcells. 11.Hemocytometerorothermeansofcountingcells. 12.Trypanblueorequivalentviabilitystain. 13.Microplatereader(540-570nmdetection)orspectrophotometer. 14.Sterilecellculturehood 15.(Optional)Graduatedocular(calibrated),orautomatedmethodforcountingstainedcellsonamembrane. 16.Shaker

ProductInformation
Components	1.FibronectinTestPlate:One24-wellcultureplate,containing12humanFN-coatedBoydenchambers,sufficientfortheevaluationof12testsamples. 2.BSAControlPlate:One24-wellcultureplate,containing12BSA-coatedBoydenchambers,sufficientfortheevaluationof12controls. 3.CellStainSolution:Onevial-10mL 4.ExtractionBuffer:Onevial-10mL 5.24-WellStainExtractionPlate 6.96-WellStainQuantitationPlate 7.Swabs:50ea 8.Forceps:1pair
Detectionmethod	FluorescentColorimetric

StorageandShippingInformation
StorageConditions	Storekitmaterialsat2°to8°Cforuptotheirexpirationdate.Donotfreeze.

Applications
Application	ThisQCM3μmEndothelialCellMigrationAssay–Fibronectin,Colorimetricisidealforthestudyofendothelialcellmigrationinresponsetoanangiogenicstimulus.
ApplicationNotes	TheMilliporeQCM™3μmEndothelialCellMigrationAssay–Fibronectin,Colorimetricisidealforthestudyofendothelialcellmigrationinresponsetoanangiogenicstimulus.Thequantitativenatureofthisassayisespeciallyusefulforlargescalescreeningofpharmacologicagents.BSA-coatedcontrolchambersprovideanappropriatemigrationcontrol.The3μmporesizeinthisassayisoptimalforendothelialcellssuchasHUVEC,butnotsufficientforfibroblastmigration.TheMilliporeQCM™3μmEndothelialCellMigrationAssay–Fibronectin,Colorimetricassayisintendedforresearchuseonly;notfordiagnosticapplications. Eachkitprovidessufficientmaterialsfortheevaluationof12samples.

BIOLOGicalInformation

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

Millipore 提供三种不同的 Immobilon PVDF 膜，是针对不同的蛋白质印迹应用的理想选择。 现在我们还 提供采用按规格裁切的印迹方片膜和两层过滤纸组成的印迹三明治。   与硝酸纤维素膜相比，Immobilon 膜具有多种优点。 它们不易破裂或卷 曲，便于裁剪且在切割过程中不会碎裂。 它们还有低背景、广泛溶剂相容性以及优异的着色能力。 此外，它们可以重复检测。 Immobilon-P 膜（沃比森-供应） ·         0.45μm 孔径 ·         建议用于大多数免疫印迹，特别在蛋 白质大于 20kDa 时 ·         Millipore 的快速免疫检测实验方法不需要膜封闭并缩短洗涤时间，将检测时间至少减少了两个小时，而不致降低灵敏 度或特异性 用于高通量实验室的印迹三明治 ·         当您需要处理多个印迹时，印迹三明治是一种便利省时的选择。 我们的印迹三明 治包含多片 Immobilon-P 膜，其中插入了按规格裁切的多张层析级印迹纸。 转印滤纸 ·         按照大多数免疫印迹尺寸进行裁切的层析级转印滤纸。 Immobilon-PSQ 膜（沃比森-供应） ·         0.2μm 孔径和大的内表面面积 ·         较其它膜具有更高的 蛋白质吸附率和测序得率 ·         建议用于小于 20kDa 的印迹蛋白质 ·         防止小分子量的蛋白质的渗漏 Immobilon-FL 膜（沃比森-供应） ·        针对荧光应用进行优化的转印膜 ·         极低的背景提高了所 有荧光检测实验的灵敏度 ·         与所有常用的荧光探针（所有激发和发射波长）兼容 ·         是多态性检测和化学荧光应用的理想选择