Description
CatalogueNumber	MAB1621
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-Neurofilament145kDaAntibody,CT,clone3H11
BackgroundInformation	NeurofilamentsareatypeofintermediatefilamentthatserveasmajorelementsoftheCytoskeletonsupportingtheaxoncytoplasm.Theyarethemostabundantfibrillarcomponentsoftheaxon,beingonaverage3-10timesmorefrequentthanaxonalmicrotubules.Neurofilaments(10nmindia.)arebuiltfromthreeintertwinedprotofibrilswhicharethemselvescomposedoftwotetramericprotofilamentcomplexsofmonomericproteins.Theneurofilamenttripletproteins(68/70,160,and200kDa)occurinboththecentralandperipheralnervoussystemandareusuallyneuronspecific.The68/70kDaNF-Lproteincanself-assembleintoafilamentousstructure,howeverthe160kDaNF-Mand200kDaNF-Hproteinsrequirethepresenceofthe68/70kDaNF-Lproteintoco-assemble.Neuromas,ganglioneuromas,gangliogliomas,ganglioneuroblastomasandneuroblastomasstainpositivelyforneurofilaments.Althoughtypicallyrestrictedtoneurons,neurofilamentshavebeendetectedinparagangliomasandadrenalandextra-adrenalpheochromocytomas.Carcinoids,neuroendocrinecarcinomasoftheskin,andoatcellcarcinomasofthelungalsoexpressneurofilaments.FormoreneurofilamentinformationseeNervousSystemCellTypeSpecificMarkerchartonlineundertheCHEMICONTechnicalSupportsection.

ProductInformation
Format	CultureSupernatant

StorageandShippingInformation
StorageConditions	Maintainat-20°Cinundilutedaliquotsforupto12months.Avoidrepeatedfreeze/thawcycles.

Applications
Application	Anti-Neurofilament145kDaAntibody,C-terminus,clone3H11detectslevelofNeurofilament145kDa&hasbeenpublished&validatedforuseinWB,IC,IH.
KeyApplications	WesternBlotting Immunocytochemistry Immunohistochemistry
ApplicationNotes	Immunohistochemistry:1:50-1:1000;freshfrozen,4%PFAfixedtissuesections Immunoblotting:1:1000-1:2000ECLdetection. Optimalworkingdilutionsmustbedeterminedbyenduser.

BIOLOGicalInformation
Immunogen	Recombinantfusionproteincontainingthelast168aminoacidsofratNF-M.PurifiedfromE.ColiinclusionbodiesbyionexchangechromatographyonDEAE-cellulosein6Murea.
Epitope	C-terminus
Clone	3H11
Host	Mouse
Specificity	Reactswithanepitopedependentonthelast84aminoacidsofratNF-M(145K).ReactsspecificallywithNF-Mfromavarietyofmammalianandavianspecies.Theantibodyisnotsensitivetothephosphorylationstateoftheneurofilament.
Isotype	IgG1
SpeciesReactivity	Avian Mammals
AntibodyType	MonoclonalAntibody
EntrezGeneNumber	NM_001105541.1 NM_005382.2
GeneSymbol	NEFM NF-M NEF3 NFM
UniProtNumber	P07197
UniProtSummary	FUNCTION:SwissProt:P07197#Neurofilamentsusuallycontainthreeintermediatefilamentproteins:L,M,andHwhichareinvolvedinthemaintenanceofneuronalcaliber. SIZE:916aminoacids;102448Da PTM:ThereareanumberofrepeatsofthetripeptideK-S-P,NFMisphosphorylatedonanumberoftheserinesinthismotif.ItisthoughtthatphosphorylationofNFMresultsintheformationofinterfilamentcrossbridgesthatareimportantinthemaintenanceofaxonalcaliber.&Phosphorylationseemstoplayamajorroleinthefunctioningofthelargerneurofilamentpolypeptides(NF-MandNF-H),thelevelsofphosphorylationbeingaltereddevelopmentallyandcoincidentwithachangeintheneurofilamentfunction. SIMILARITY:SwissProt:P07197##Belongstotheintermediatefilamentfamily.

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

Millipore 提供三种不同的 Immobilon PVDF 膜，是针对不同的蛋白质印迹应用的理想选择。 现在我们还 提供采用按规格裁切的印迹方片膜和两层过滤纸组成的印迹三明治。   与硝酸纤维素膜相比，Immobilon 膜具有多种优点。 它们不易破裂或卷 曲，便于裁剪且在切割过程中不会碎裂。 它们还有低背景、广泛溶剂相容性以及优异的着色能力。 此外，它们可以重复检测。 Immobilon-P 膜（沃比森-供应） ·         0.45μm 孔径 ·         建议用于大多数免疫印迹，特别在蛋 白质大于 20kDa 时 ·         Millipore 的快速免疫检测实验方法不需要膜封闭并缩短洗涤时间，将检测时间至少减少了两个小时，而不致降低灵敏 度或特异性 用于高通量实验室的印迹三明治 ·         当您需要处理多个印迹时，印迹三明治是一种便利省时的选择。 我们的印迹三明 治包含多片 Immobilon-P 膜，其中插入了按规格裁切的多张层析级印迹纸。 转印滤纸 ·         按照大多数免疫印迹尺寸进行裁切的层析级转印滤纸。 Immobilon-PSQ 膜（沃比森-供应） ·         0.2μm 孔径和大的内表面面积 ·         较其它膜具有更高的 蛋白质吸附率和测序得率 ·         建议用于小于 20kDa 的印迹蛋白质 ·         防止小分子量的蛋白质的渗漏 Immobilon-FL 膜（沃比森-供应） ·        针对荧光应用进行优化的转印膜 ·         极低的背景提高了所 有荧光检测实验的灵敏度 ·         与所有常用的荧光探针（所有激发和发射波长）兼容 ·         是多态性检测和化学荧光应用的理想选择