Description
CatalogueNumber	GF094
Replaces	GF025
BrandFamily	Chemicon®
TradeName	Chemicon
Description	VascularEndothelialGrowthFactor,Recombinanthuman,165aaisoform
Overview	VascularEndothelialCellGrowthFactor(VEGF)isasecretedhomodimericproteinsecretedbyavarietyofvascularizedtissues.ThereportedactivitiesofVEGFincludestimulationofendothelialcellgrowth,angiogenesis,andcapillarypermeABIlity.HumanVEGFisa38.2kDahomodimericproteinconsistingoftwo165aminoacidpolypeptidechains.
AlternateNames	VEGF

ProductInformation
Presentation	Lyophilizedfromsterilefiltered20mMaceticacid,containingnoadditives.

StorageandShippingInformation
StorageConditions	Thelyophilizedpowderisstableforafewweeksatroomtemperature,butbeststoredat-20°Cforuptooneyearfromdateofreceipt.Afteraquickspin,reconstitutewithsterilewatertoaconcentrationof0.1-1.0mg/mL.Reconstitutedmaterialcanbestoredat2°C-8°Cforoneweek,butundilutedaliquotsat-20°Carerecommended.Avoidrepeatedfreeze/thawcycles.

Applications
Application	VascularEndothelialCellGrowthFactor(VEGF)isasecretedhomodimericproteinsecretedbyavarietyofvascularizedtissues.
KeyApplications	CellCulture
ApplicationNotes	Formostinvitroapplications,humanVEGFexertsitsBIOLOGicalactivityintheconcentrationrangeof0.1to50.0ng/mL.Respondingcellsare(partiallist):Endothelialcells.

BiologicalInformation
Purity	Greaterthan98%bySDS-PAGE.Endotoxinlevelislessthan0.1ngperμg(1EU/ug)ofVEGF
Source	E.coli
SpecificActivity	ThebiologicalactivityofMillipore'shumanVEGFwasdeterminedbyitsmitogenicactivityonhumanumbilicalveinendothelialcells(HUVEC).Mitogenicactivitywasobtainedinaconcentrationrangeof1.0-8.0ng/mL.
EntrezGeneNumber	NM_001025366.1 NM_001025367.1 NM_001025368.1 NM_001025369.1 NM_001025370.1 NM_001033756.1 NM_003376.4
EntrezGeneSummary	ThisgeneisamemberofthePDGF/VEGFgrowthfactorfamilyandencodesaproteinthatisoftenfoundasadisulfidelinkedhomodimer.Thisproteinisaglycosylatedmitogenthatspecificallyactsonendothelialcellsandhasvariouseffects,includingmediatingincreasedvascularpermeability,inducingangiogenesis,vasculogenesisandendothelialcellgrowth,promotingcellmigration,andinhibitingapoptosis.ElevatedlevelsofthisproteinislinkedtoPOEMSsyndrome,alsoknownasCrow-Fukasesyndrome.Mutationsinthisgenehavebeenassociatedwithproliferativeandnonproliferativediabeticretinopathy.Alternativelysplicedtranscriptvariants,encodingeitherfreelysecretedorcell-associatedisoforms,havebeencharacterized.Thereisalsoevidencefortheuseofnon-AUG(CUG)translationinitiationsitesupstreamof,andin-framewiththefirstAUG,leADIngtoadditionalisoforms.
GeneSymbol	VEGFA MGC70609 VPF VEGF VEGF-A
UniProtNumber	P15692
UniProtSummary	FUNCTION:SwissProt:P15692#Growthfactoractiveinangiogenesis,vasculogenesisandendothelialcellgrowth.Inducesendothelialcellproliferation,promotescellmigration,inhibitsapoptosis,andinducespermeabilizationofbloodvessels.BindstotheVEGFR1/Flt-1andVEGFR2/Kdrreceptors,heparansulfateandheparin.Neuropilin-1bindsisoformsVEGF-165andVEGF-145. SIZE:232aminoacids;27042Da SUBUNIT:Homodimer;disulfide-linked.AlsofoundasheterodimerwithPlGF(Bysimilarity). SUBCELLULARLOCATION:Secreted.Note=VEGF121isacidicandfreelysecreted.VEGF165ismorebasic,hasheparin-bindingpropertiesand,althoughasignicantproportionremainscell-associated,mostisfreelysecreted.VEGF189isverybasic,itiscell-associatedaftersecretionandisboundavidlybyheparinandtheextracellularmatrix,althoughitmaybereleasedasasolubleformbyheparin,heparinaseorplasmin. TISSUESPECIFICITY:TheVEGF189,VEGF-165andVEGF-121isoformsarewidelyexpressed,whereastheVEGF206andVEGF-145areuncommon. SIMILARITY:SwissProt:P15692##BelongstothePDGF/VEGFgrowthfactorfamily.
StemCellType	NeuralStemCells MesenchymalStemCells HematopoieticStemCells
MolecularWeight	38.2kDa

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

Millipore 提供三种不同的 Immobilon PVDF 膜，是针对不同的蛋白质印迹应用的理想选择。 现在我们还 提供采用按规格裁切的印迹方片膜和两层过滤纸组成的印迹三明治。   与硝酸纤维素膜相比，Immobilon 膜具有多种优点。 它们不易破裂或卷 曲，便于裁剪且在切割过程中不会碎裂。 它们还有低背景、广泛溶剂相容性以及优异的着色能力。 此外，它们可以重复检测。 Immobilon-P 膜（沃比森-供应） ·         0.45μm 孔径 ·         建议用于大多数免疫印迹，特别在蛋 白质大于 20kDa 时 ·         Millipore 的快速免疫检测实验方法不需要膜封闭并缩短洗涤时间，将检测时间至少减少了两个小时，而不致降低灵敏 度或特异性 用于高通量实验室的印迹三明治 ·         当您需要处理多个印迹时，印迹三明治是一种便利省时的选择。 我们的印迹三明 治包含多片 Immobilon-P 膜，其中插入了按规格裁切的多张层析级印迹纸。 转印滤纸 ·         按照大多数免疫印迹尺寸进行裁切的层析级转印滤纸。 Immobilon-PSQ 膜（沃比森-供应） ·         0.2μm 孔径和大的内表面面积 ·         较其它膜具有更高的 蛋白质吸附率和测序得率 ·         建议用于小于 20kDa 的印迹蛋白质 ·         防止小分子量的蛋白质的渗漏 Immobilon-FL 膜（沃比森-供应） ·        针对荧光应用进行优化的转印膜 ·         极低的背景提高了所 有荧光检测实验的灵敏度 ·         与所有常用的荧光探针（所有激发和发射波长）兼容 ·         是多态性检测和化学荧光应用的理想选择