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蚂蚁淘在线 / 品牌中心 / Discovery / Discovery Antibodies/Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody/25 µl/AAR-011-25 µl

Discovery Antibodies/Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody/25 µl/AAR-011-25 µl

价格
￥4940.00
货号：AAR-011-25µl
浏览量：107
品牌：Discovery
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商品描述
Cat#:AAR-011
AlternativeNameAT1Receptor,AT1R,AGTR1
  • LyophilizedPowder
  • AntigenIncl.
    • Type:Polyclonal
    Host:Rabbit
    Reactivity:h,m,r
      • PeptideNSSTEDGIKRIQDDC,correspondingtoaminoacidresidues4-18ofhumanAT1receptor(AccessionP30556).Extracellular,N-terminus.
        Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody
    Accession(Uniprot)NumberP30556
    GeneID185
    PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
    HomologyMouse,rat,rabbit,dog-identical.
    RRIDAB_2040271.
    PurityAffinitypurifiedonimmobilizedantigen.
    FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
    IsotypeRabbitIgG.
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution25µl,50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
    Antibodyconcentrationafterreconstitution0.7mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
    NegativecontrolantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
    Negativecontrolantigenreconstitution100µldoubledistilledwater(DDW).
    Negativecontrolantigenstorageafterreconstitution-20°C.
    PreadsorptionControl1μgpeptideper1μgantibody.
    StandardqualitycontrolofeachlotWesternblotanalysis.
    Applications:ic,if,ifc,ih,lci,wb
    Mayalsoworkin:ip*
      • Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody
        Westernblotanalysis ofmousekidney(lanes1and3)andmouseheart(lanes2and4)membranes:
        1,2.Anti-AngiotensinIIReceptorType-1(extracellular) Antibody(#AAR-011),(1:500).
        3,4.Anti-AngiotensinIIReceptorType-1(extracellular)Antibody,preincubatedwiththenegativecontrolantigen.
        Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody
        Westernblotanalysis ofratliver(lanes1and3)andratkidney(lanes2and4)membranes:
        1,2.Anti-AngiotensinIIReceptorType-1(extracellular)Antibody(#AAR-011),(1:200).
        3,4.Anti-AngiotensinIIReceptorType-1(extracellular)Antibody,preincubatedwiththenegativecontrolantigen.
      • Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody
        Expressionof AngiotensinIIReceptorType-1inmousecerebellum
        ImmunohistochemicalstainingofmousecerebellumusingAnti-AngiotensinIIReceptor Type-1(extracellular) Antibody(#AAR-011). A. Mouseanti-Parvalbumin(red)isdetectedinthePurkinjelayer.B.Inthesamesection,AT1receptor(green)isalsopresentinthePurkinjelayer.ArrowspointatAT1receptorimmunoreactivecells.MergeofAandBpanels revealspartialco-localization.
      • Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody
        CellsurfacedetectionofAGTR1 inliveintact humanJurkatT-cellleukemiacells:
        ___Unstained cells+goat-anti-rabbit-FITC.
        ___Cells+Anti-AngiotensinIIReceptorType-1 (extracellular) Antibody(#AAR-011),(5µg)+goat-anti-rabbit-FITC.
        ___Cells+Anti-AngiotensinIIReceptorType-1 (extracellular)Antibody,(10µg)+goat-anti-rabbit-FITC.
        Thenegativecontrolantigenisnotsuitableforthisapplication.
      • Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody
        ExpressionofAGTR1inratC6gliomacells
        Cellsurfacedetectionof AGTR1inliveintactratC6gliomacells. A.CellswerestainedusingAnti-AngiotensinIIReceptorType-1 (extracellular) Antibody(#AAR-011)(1:100),followedbygoatanti-rabbit-AlexaFluor-555secondaryantibody.B.LiveintactC6cells.
    References
    • 1.Sasaki,K.etal.(1991)Nature351,230.
    • 2.Murphy,T.J.etal.(1991)Nature351,233.
    • 3.DeGasparo,M.etal.(2000)Pharmacol.Rev.52,415.
    • 4.Hunyady,L.andCatt,K.J.(2006)Mol.Endocrinol.20,953.

      • AngiotensinIIreceptortype1(AT1 receptor,AGTR1)isoneofthereceptorsthatbindstheoctapeptidehormoneAngiotensinII(AngII).
         
        AngIIisthepeptidehormonethatgeneratesmostoftheknowneffectsoftherenin-angiotensinsystem(RAS).AngIIisgeneratedfromtheangiotensinogenproteinbytheactionsofrenin,angiotensinconvertingenzyme(ACE)andotherpeptidases.AngIIhasacentralroleincardiovascularhomeostasisbyregulatingvasoconstriction,renalNa+ andwaterreadsorption.Inaddition,AngIIinducescellgrowthandproliferationandhaspro-inflammatoryeffects.
         
        MostofthephysiologicalactionsofAngIIaremediatedbyAT1 receptoramemberofthe7-transmembranedomain,Gprotein-coupledreceptor(GPCR)superfamily.

        TheAT1 receptoriscoupledtoaGq/11 proteinthatactivatesphospholipaseC(PLC)andleadstoproductionofinositol1,4,5-trisphosphate(IP3)andintracellularCa2+ mobilization.InadditiontotherapidactionsmediatedbyIP3 andCa2+ signaling,AT1 receptorelicitsothersignalingresponsesincludingactivationoftheMAPKandJak/STAT.Together,thesesignalingpathwaysmediatemostofAngIIcellularresponsesthatinclude,controlofhormonesecretion,regulationofcellgrowthandapoptosis,regulationofionchannelsactivationandcellmigration.
         
        InaccordancewiththepleiotropicactionsofAngII,theAT1 receptorhasawidedistributionwithhighlevelsdetectedintheadrenalgland,kidney,brain,heart,liver,etc.
         
        Asisthecasewithmanyotherpeptidereceptors,theAT1 receptorundergoesligand-inducedendocytosis,aprocessthatcontributestoreceptordesensitizationandsequestrationandcontributestoregulatereceptorsignaling.

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,
    IF-Immunofluorescence,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat
    LyophilizedPowderNegativeControlAntigenIncluded
    receivea25µlfreetrialsample!
    Image&Title:

    Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody
    ExpressionofAT1Rinmousesatellitecellsandmousemyofibers.A.Indirectflowcytometryofmousesatellitecellsusing Anti-AngiotensinII ReceptorType-1(extracellular) Antibody(#AAR-011).B.ImmunocytochemicalstainingofintactculturedmousemyofibersusingsameantibodyasinA.Adaptedfrom Yoshida,T. etal. (2013)J.Biol.Chem.288,23823.withpermissionoftheAmericanSocietyforBiochemistryandMolecularBIOLOGy.

    Lastupdate:10/11/2019

    Anti-AngiotensinIIReceptorType-1(extracellular)Antibody(#AAR-011)isahighlyspecificantibodydirectedagainstanepitopeofthehumanprotein.Theantibodycanbeusedinwesternblot,immunohistochemistry,immunocytochemistry,andindirectflowcytometryapplications.IthasbeendesignedtorecognizeAT1receptorfromrat,mouse,andhumansamples.

    Forresearchpurposesonly,notforhumanuse
    Discovery Antibodies的Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.CDK的活动受到严格控制。CDK2活性可以被包括P21在内的一系列抑制剂抑制。细胞周期失调和增殖控制的丧失与细胞转化和癌症密切相关。CDK2在DNA损伤反应（DDR）中的细胞周期停滞中也似乎起着积极作用，它的抑制作用会阻碍DDR，并使细胞对电离辐射敏感，从而诱导细胞死亡。该抗体与AlexaFluor®488偶联。AlexaFluor®488是一种流行的鲜绿色荧光染料，具有高pH稳定性。

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