Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
ThisantibodyisshippedwithitsantigenFREEofcharge!
- PeptideNSSTEDGIKRIQDDC,correspondingtoaminoacidresidues4-18ofhumanAT1receptor(AccessionP30556).Extracellular,N-terminus.
- Westernblotanalysis ofmousekidney(lanes1and3)andmouseheart(lanes2and4)membranes:1,2.Anti-AngiotensinIIReceptorType-1(extracellular) Antibody(#AAR-011),(1:500).
3,4.Anti-AngiotensinIIReceptorType-1(extracellular)Antibody,preincubatedwiththenegativecontrolantigen.Westernblotanalysis ofratliver(lanes1and3)andratkidney(lanes2and4)membranes:1,2.Anti-AngiotensinIIReceptorType-1(extracellular)Antibody(#AAR-011),(1:200).
3,4.Anti-AngiotensinIIReceptorType-1(extracellular)Antibody,preincubatedwiththenegativecontrolantigen.
- Expressionof AngiotensinIIReceptorType-1inmousecerebellumImmunohistochemicalstainingofmousecerebellumusingAnti-AngiotensinIIReceptor Type-1(extracellular) Antibody(#AAR-011). A. Mouseanti-Parvalbumin(red)isdetectedinthePurkinjelayer.B.Inthesamesection,AT1receptor(green)isalsopresentinthePurkinjelayer.ArrowspointatAT1receptorimmunoreactivecells.MergeofAandBpanels revealspartialco-localization.
- CellsurfacedetectionofAGTR1 inliveintact humanJurkatT-cellleukemiacells:___Unstained cells+goat-anti-rabbit-FITC.
___Cells+Anti-AngiotensinIIReceptorType-1 (extracellular) Antibody(#AAR-011),(5µg)+goat-anti-rabbit-FITC.
___Cells+Anti-AngiotensinIIReceptorType-1 (extracellular)Antibody,(10µg)+goat-anti-rabbit-FITC.Thenegativecontrolantigenisnotsuitableforthisapplication.
- ExpressionofAGTR1inratC6gliomacellsCellsurfacedetectionof AGTR1inliveintactratC6gliomacells. A.CellswerestainedusingAnti-AngiotensinIIReceptorType-1 (extracellular) Antibody(#AAR-011)(1:100),followedbygoatanti-rabbit-AlexaFluor-555secondaryantibody.B.LiveintactC6cells.
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AngiotensinIIreceptortype1(AT1 receptor,AGTR1)isoneofthereceptorsthatbindstheoctapeptidehormoneAngiotensinII(AngII).
AngIIisthepeptidehormonethatgeneratesmostoftheknowneffectsoftherenin-angiotensinsystem(RAS).AngIIisgeneratedfromtheangiotensinogenproteinbytheactionsofrenin,angiotensinconvertingenzyme(ACE)andotherpeptidases.AngIIhasacentralroleincardiovascularhomeostasisbyregulatingvasoconstriction,renalNa+ andwaterreadsorption.Inaddition,AngIIinducescellgrowthandproliferationandhaspro-inflammatoryeffects.
MostofthephysiologicalactionsofAngIIaremediatedbyAT1 receptoramemberofthe7-transmembranedomain,Gprotein-coupledreceptor(GPCR)superfamily.
TheAT1 receptoriscoupledtoaGq/11 proteinthatactivatesphospholipaseC(PLC)andleadstoproductionofinositol1,4,5-trisphosphate(IP3)andintracellularCa2+ mobilization.InadditiontotherapidactionsmediatedbyIP3 andCa2+ signaling,AT1 receptorelicitsothersignalingresponsesincludingactivationoftheMAPKandJak/STAT.Together,thesesignalingpathwaysmediatemostofAngIIcellularresponsesthatinclude,controlofhormonesecretion,regulationofcellgrowthandapoptosis,regulationofionchannelsactivationandcellmigration.
InaccordancewiththepleiotropicactionsofAngII,theAT1 receptorhasawidedistributionwithhighlevelsdetectedintheadrenalgland,kidney,brain,heart,liver,etc.
Asisthecasewithmanyotherpeptidereceptors,theAT1 receptorundergoesligand-inducedendocytosis,aprocessthatcontributestoreceptordesensitizationandsequestrationandcontributestoregulatereceptorsignaling.
ExpressionofAT1Rinmousesatellitecellsandmousemyofibers.A.Indirectflowcytometryofmousesatellitecellsusing Anti-AngiotensinII ReceptorType-1(extracellular) Antibody(#AAR-011).B.ImmunocytochemicalstainingofintactculturedmousemyofibersusingsameantibodyasinA.Adaptedfrom Yoshida,T. etal. (2013)J.Biol.Chem.288,23823.withpermissionoftheAmericanSocietyforBiochemistryandMolecularBIOLOGy.
Anti-AngiotensinIIReceptorType-1(extracellular)Antibody(#AAR-011)isahighlyspecificantibodydirectedagainstanepitopeofthehumanprotein.Theantibodycanbeusedinwesternblot,immunohistochemistry,immunocytochemistry,andindirectflowcytometryapplications.IthasbeendesignedtorecognizeAT1receptorfromrat,mouse,andhumansamples.