品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Lyophilized Powder Lyophilized Powder
This product is freeze dried. All water molecules have been removed.
Bioassay Tested Bioassay Tested
Every lot is tried & tested in a relevant biological assay.
Our Bioassay
Source Synthetic
MW: 356.3
Purity: >99% (HPLC)
Effective concentration 1-5 μM.
Chemical name 1,4-Dihydro-2,6-dimethyl-5-nitro-4-[2-(trifluoromethyl) phenyl]-3-pyridinecarboxylic acid, methyl ester.
Molecular formula C16H15F3N2O4.
CAS No.: 71145-03-4, 93468-89-4.
Activity (±)-Bay K8644 is a dihydropyridine that acts as an L-type, voltage-gated Ca2+ channel agonist. The (-)-enantiomer has strong Ca2+ channel agonist properties, whereas the (+)-enantiomer has weak Ca2+ channel antagonist activity. The net activity of the racemic mix is L-type channel opening. In the presence of this agonist, channels tend to open for longer periods causing a large increase in the channel microscopic response1-4. (±)-Bay K8644 is also an inhibitor (by increasing the inactivation) of a Shaker KV channel5.
-
- Hess, P. et al. (1984) Nature 311, 538.
- Nowycky, M.C. et al. (1985) Proc. Natl. Acad. Sci. U.S.A. 82, 2178.
- Bechem, M. and Hoffmann, H. (1993) Pflugers Arch. 424, 343.
- Triggle, D.J. and Rampe, D. (1989) Trends Pharmacol. Sci. 10, 507.
- Avdonin, V. et al. (1997) J. Gen. Physiol. 109, 169.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility Ethanol, methanol or DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage of solutions Up to four weeks at 4°C or three months at -20°C.
-
Alomone Labs (±)-Bay K8644 increases L-type Ca2+ currents expressed in Xenopus oocytes.Left: I-V relation before (squares) and during (circles) bath perfusion of 5 μM (±)-Bay K8644 (#B-350). Right: An example of current response to 200 ms depolarization to +20mV (from holding potential of -100 mV) before (black) and during (pink) perfusion of (±)-Bay K8644.
Alomone Labs (±)-Bay K8644 modulates the phosphorylation of ERK1/2 MAPK in Jurkat T cells.Cells were preincubated with or without EGTA or Nifedipine for 20 min then stimulated with (±)-Bay K8644 (#B-350) or with Ionomycin for 10 min. The cell proteins were blotted and probed with anti-phospho ERK1/2 (upper panel) or with anti-ERK1/2 (lower panel).
References - Scientific background
- 1. Hess, P. et al. (1984) Nature 311, 538.
- 2. Nowycky, M.C. et al. (1985) Proc. Natl. Acad. Sci. U.S.A. 82, 2178.
- 3. Bechem, M. and Hoffmann, H. (1993) Pflugers Arch. 424, 343.
-
(±)-Bay K8644 is an opener of all L-type channels (CaV1 family). In the presence of this agonist, channels tend to open for longer periods causing a large increase in the channel macroscopic response.
The net activity of the racemic mix is that of the (-)-enantiomer agonistic activity.
(±)-Bay K8644 is a widely used L-type Ca2+ channel activator in various neuronal, muscle, endocrine, thyroid and other cell types.1-3
Target L-type Ca2+ channels
Lyophilized Powder
Click here to receive a 5 mg free trial sample!
Last update: 24/09/2019
(±)-Bay K8644 (#B-350) is a highly pure, synthetic, and biologically active compound.
For research purposes only, not for human use
Discovery Antibodies的Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.CDK的活动受到严格控制。CDK2活性可以被包括P21在内的一系列抑制剂抑制。细胞周期失调和增殖控制的丧失与细胞转化和癌症密切相关。CDK2在DNA损伤反应（DDR）中的细胞周期停滞中也似乎起着积极作用，它的抑制作用会阻碍DDR，并使细胞对电离辐射敏感，从而诱导细胞死亡。该抗体与AlexaFluor®488偶联。AlexaFluor®488是一种流行的鲜绿色荧光染料，具有高pH稳定性。
