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蚂蚁淘在线 / 品牌中心 / Discovery / Discovery Antibodies/Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody/50 µl/APC-101-B-50 µl

Discovery Antibodies/Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody/50 µl/APC-101-B-50 µl

价格
￥10540.00
货号：APC-101-B-50µl
浏览量：127
品牌：Discovery
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商品描述
Cat#:APC-101-B
AlternativeNamePotassiumvoltage-gatedchannelsubfamilyAmember3
  • LyophilizedPowder
  • AntigenIncl.
    • Type:Polyclonal
    Host:Rabbit
    Reactivity:h,m,r
      • PeptideKDYPASTSQDSFEA(C),correspondingtoaminoacidresidues263-276ofhumanKV1.3(AccessionP22001).ExtracellularloopbetweendomainsS1andS2.
        Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody
    Accession(Uniprot)NumberP22001
    GeneID3738
    PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
    HomologyRat,mouse -12/14aminoacidresiduesidentical.
    RRIDAB_2341030.
    PurityAffinitypurifiedonimmobilizedantigen.
    FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
    IsotypeRabbitIgG.
    LabelBiotin.
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution50µldoubledistilledwater(DDW).
    Antibodyconcentrationafterreconstitution1mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
    NegativecontrolantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
    Negativecontrolantigenreconstitution100µldoubledistilledwater(DDW).
    Negativecontrolantigenstorageafterreconstitution-20°C.
    PreadsorptionControl1µgpeptideper1µgantibody.
    StandardqualitycontrolofeachlotWesternblotanalysis.
    Applications:fc,if,ih,wb
    Mayalsoworkin:ic*,ip*,lci*
      • Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody
        WesternblotanalysisofhumanJurkatTcellleukemiacelllysate(lanes1and3)andmousebrainlysate(lanes2and4):
        1-2. Anti-KV1.3(KCNA3)(extracellular)-BiotinAntibody(#APC-101-B),(1:1000).
        3-4.Anti-KV1.3(KCNA3)(extracellular)-BiotinAntibody,preincubatedwiththecontrolpeptideantigen.
      • Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody
        Immuno-colocalizationofKV1.3andKV1.5inmousecerebellum
        Immunohistochemicalstainingofmouseperfusion-fixedfrozenbrainsectionsusing Anti-KV1.3(KCNA3)(extracellular)-Biotin Antibody(#APC-101-B)(1:400),and Anti-KV1.5(KCNA5)-ATTO-550Antibody (#APC-004-AO),(1:60).A.KV1.3staining(green)isdetectedinBergmanngliasomaandprocessesinthemolecularlayer(Mol)(arrow).B.SamesectionshowsstainingforKV1.5(red).C.Mergeofthetwoimagessuggestsconsiderableco-localizationinthesomaofBergmannglia.Inthemolecularlayer,thedistributionofKV1.5isdiffuse,unlikethediscretestainingforKV1.3inglialprocesses.CellnucleiwerevisualizedwithDAPI(blue).
      • Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody
        CellsurfacedetectionofKV1.3inliveintacthumanJurkat Tcellleukemiacellline:
        ___ Cells+Streptavidin-Alexa647.
        ___ Cells+ Anti-KV1.3(KCNA3)(extracellular)-BiotinAntibody(#APC-101-B),(1:10)+Streptavidin-Alexa647.
        Thecontrolantigenisnotsuitableforthisapplication.
    References
    • 1.Chandy,K.G.etal.(2001)Toxicon39,1269.
    • 2.Koo,G.C.etal.(1997)J.Immunol.158,5120.

      • KV1.3belongstothe Shaker familyofvoltage-dependentK+ channels. Thechannel,encodedby KCNA3,iswidelyexpressedinthebrain,lungandosteoclastsandinseveralcell populationsofhematopoieticorigin.TheprominenceofKV1.3channelsinthesecells(particularlyinTlymphocytes)directedmuchresearch attention.ItwasfoundthatKV1.3isthemainchannelresponsIBLeformaintainingtherestingpotentialinquiescentcellsandregulatingthe Ca2+ signalingthatisindispensablefornormalTlymphocyteactivation.1,2 BasedonthecentralroleofKV1.3inregulatingtheinitiationofanimmuneresponse, thechannelhasbeenrecognizedasapotentialtargetforimmunosuppressantdrugs.1ThecentralroleofKV1.3inimmunesystemcellscreatedarealneedforaspecific antibodythatwouldbeabletoworkinflowcytometryapplications.

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IF-Immunofluorescence,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat
    LyophilizedPowderNegativeControlAntigenIncluded
    Lastupdate:16/01/2020

    Anti-KV1.3(KCNA3)(extracellular)Antibody(#APC-101)isahighlyspecificantibodydirectedagainstanextracellularepitopeofthehumanprotein.Theantibodycanbeusedinwesternblot,immunohistochemistry,livecellimaging,andindirectflowcytometryapplications.IthasbeendesignedtorecognizeKV1.3potassiumchannelfromhuman,mouse,andratsamples.

    Anti-KV1.3(KCNA3)(extracellular)-BiotinAntibody(#APC-101-B)isdirectlylabeledwithbiotin.StrepavidintaggedwithHRPorwithafluorescentprobecanthenbeusedtodetecttheprotein.Thebiotin/strepavidinsystemisidealforminimizingcross-reactivitywhensamespeciesantibodiesaresimultaneouslyused.Anti-KV1.3(KCNA3)(extracellular)-BiotinAntibody hasbeentestedinwesternblot,immunohistochemistryanddirectflowcytometryandisspeciallysuitedtoexperimentsrequiringsimultaneouslabelingofdifferentMarkers.

    Forresearchpurposesonly,notforhumanuse
    Discovery Antibodies的Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.CDK的活动受到严格控制。CDK2活性可以被包括P21在内的一系列抑制剂抑制。细胞周期失调和增殖控制的丧失与细胞转化和癌症密切相关。CDK2在DNA损伤反应（DDR）中的细胞周期停滞中也似乎起着积极作用，它的抑制作用会阻碍DDR，并使细胞对电离辐射敏感，从而诱导细胞死亡。该抗体与AlexaFluor®488偶联。AlexaFluor®488是一种流行的鲜绿色荧光染料，具有高pH稳定性。

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