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- Peptide (C)EAMNHDLELGHTHG, corresponding to amino acid residues 252-265 of human CALHM1 (Accession Q8IU99).
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Expression of CALHM1 in rat hippocampusImmunohistochemical staining of rat hippocampus using Anti-CALHM1-ATTO-594 Antibody (#ACC-101-AR). A. Staining of CALHM1 appears only in the hippocampal CA1 pyramidal layer. B. Nuclear staining using DAPI as the counterstain. C. Merge of A and B.
Immuno-colocalization of GluN1 and CALHM1 in mouse hippocampal CA1 regionImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGC-001), (1:200) and Anti-CALHM1-ATTO-594 Antibody (#ACC-101-AR), (1:60). A. Sections were stained with Anti-NMDAR1 (GluN1) (extracellular) Antibody, followed by goat-anti-rabbit-Cy2 (green). Staining reveals expression in neurons of the pyramidal layer (an arrow points at the layer). B. The same section was incubated with Anti-CALHM1-ATTO-594 Antibody, (red). C. Merge of the two images demonstrates colocalization of GluN1 and CALHM1 in pyramidal neurons.
- 1. Green, K.N. and LaFerla, F.M. (2008) Neuron 59, 190.
- 2. Koppel, J. et al. (2011) Mol. Med. 17, 974.
- 3. Dreses-Werringloer, U. et al. (2008) Cell 133, 1149.
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A misbalance in Ca2+ homeostasis seems to be related to the development of Alzheimer’s disease (AD). Indeed Neuronal Ca2+ balance may affect the levels of proteins associated with AD, such as amyloid-beta (Ab) and tau1.
Calcium homeostasis modulator 1 (CALHM1) is a cerebral Ca2+ channel component responsible for controlling intracellular Ca2+ levels and Ab metabolism2,3. CALHM1 is a three transmembrane glycoprotein, mostly localized to the endoplasmic reticulum (ER) although it can be detected at the plasma membrane. A functional channel is formed by multiple subunits which has some structural similarities to the NMDA receptor. It is expressed in all brain regions1.
In cultured cells, overexpression of CALHM1 increases intracellular Ca2+ levels and reduces Ab accumulation, thereby reinforcing its implication in the development of AD. Indeed, a polymorphism in the gene was found to affect the onset of AD2.
Anti-CALHM1 Antibody (#ACC-101) is a highly specific antibody directed against an epitope of the human calcium homeostasis modulator protein 1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CALHM1 from rat, mouse, and human samples.
Anti-CALHM1-ATTO-594 Antibody (#ACC-101-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-CALHM1-ATTO-594 Antibody has been tested in immunohistochemistry applications and is especially suited for experiments requiring simultaneous labeling of different markers.
