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Discovery Antibodies/Anti-Calcitonin Receptor (extracellular) Antibody/25 µl/ACR-053-25 µl

价格
¥4940.00
货号:ACR-053-25µl
浏览量:127
品牌:Discovery
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商品描述
Cat#:ACR-053
AlternativeNameCALCR,CTreceptor,CT-R,C1A/C1B
  • LyophilizedPowder
  • AntigenIncl.
  • Type:Polyclonal
    Host:Rabbit
    Reactivity:m,r
    Mayalsoworkin:h*
      • Peptide(C)HLVEVVPNGDLVRRD,correspondingtoaminoacidresidues201-215ofratCALCR(Accession P32214).
        Anti-Calcitonin Receptor (extracellular) Antibody
    Accession(Uniprot)NumberP32214
    GeneID116506
    PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
    HomologyMouse–identical;human–14/15aminoacidresiduesidentical.
    RRIDAB_2756577.
    PurityAffinitypurifiedonimmobilizedantigen.
    FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
    IsotypeRabbitIgG.
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
    Antibodyconcentrationafterreconstitution0.85mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
    NegativecontrolantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
    Negativecontrolantigenreconstitution100µldoubledistilledwater(DDW).
    Negativecontrolantigenstorageafterreconstitution-20°C.
    PreadsorptionControl1µgpeptideper1µgantibody.
    StandardqualitycontrolofeachlotWesternblotanalysis.
    Applications:ic,if,lci,wb
    Mayalsoworkin:ifc*,ih*,ip*
      • Anti-Calcitonin Receptor (extracellular) Antibody
        Westernblotanalysisofratbrainmembrane(lanes1and3)andmousebrainlysate(lanes2and4):
        1,2. Anti-CalcitoninReceptor(extracellular) Antibody(#ACR-053),(1:200).
        3,4.Anti-CalcitoninReceptor(extracellular)Antibody,preincubatedwiththenegativecontrolantigen.
      • Anti-Calcitonin Receptor (extracellular) Antibody
        ExpressionofCALCRinratPC12cells
        Cellsurfacedetectionof CALCRinintactlivingratpheochromocytoma(PC12)cells.A,C.Extracellularstainingofcellsusing Anti-CalcitoninReceptor(extracellular) Antibody,(#ACR-053),(1:50).B,D.MergedimagesofAandC,eachwithitsrespectiveliveviewofthecells.
    References
    • 1.Segovia-Silvestre,T.etal.(2011)BMCRes.Notes4,407.
    • 2.Barwell,J.etal.(2012)Br.J.Pharmacol.166,51.
    • 3.Taboulet,J.etal.(1998)Hum.Mol.Genet.7,2129.
    • 4.Gorn,A.H.etal.(1995)J.Clin.Invest.95,2680.
    • 5.Gorn,A.H.etal.(1992)J.Clin.Invest.90,1726.
    • 6.Gooi,J.H.etal.(2014)J.Endocrinol.221,181.
      • thecalcitoninreceptor(CTR)isaclassB G-proteincoupledreceptor (GPCR)thatsignalsviaactivationofcAMPandproteinkinaseC(PKC).Itisoneof15humanclassBGPCRs1,2.ApartfromCTR,calcitoninreceptor-likereceptor(CTLR)istheonlyotherB-classGPCRtobindcalcitonin.Todate,therearethreeknownhumanCTRmRNAisoformsarisingfromalternativesplicing3.

        Calcitoninitselfisapeptidehormonethatcontributestobonecalcificationbydirectinhibitionofosteoclastswhichresorbthebonytissue.Itispartofasix-memberfamilythathasvariedfunctionalities.Bybindingtoreceptoractivitymodifyingproteins(RAMPs),CTRandCTLRcanformreceptorstoeverymemberofthecalcitoninfamily.Forinstance,RAMP1andCTRconstructthecalcitoningene-relatedpeptide(CGRP)receptor.

        BothCTRandCTLRhaveseventransmembranedomains,anintracellularC-terminal,andanextracellularN-terminal.Toactivatethereceptors,theirN-terminalmustfirstcapturetheC-terminaloftheligandandthencarriedtothetransmembraneloopswhereitbestowsaconformationalchangeuponthem.

        Besidesthebone,CTRhasprominentrolesinthekidney,CNS,placenta,ovaries,sperm,lung,andinthebrain4,5.
        Inosteoclasts,theexpressionofCTRsdeclineswithage.Interestingly,polymorphismoftheCTRgeneisstronglyassociatedwithdecreasedfracture-riskinpost-menopausalwomen3,6.

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,
    IF-Immunofluorescence,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat
    receivea25µlfreetrialsample!
    Lastupdate:10/11/2019

    AlomoneLabsispleasedtoofferahighlyspecificantibodydirectedagainstanepitopeofratCT-R.Anti-CalcitoninReceptorAntibody(#ACR-053)canbeusedinwesternblotandlivecellimagingapplications.IthasbeendesignedtorecognizeCT-Rfromhuman,ratandmousesamples.

    Forresearchpurposesonly,notforhumanuse
    Discovery Antibodies的Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.CDK的活动受到严格控制。CDK2活性可以被包括P21在内的一系列抑制剂抑制。细胞周期失调和增殖控制的丧失与细胞转化和癌症密切相关。CDK2在DNA损伤反应(DDR)中的细胞周期停滞中也似乎起着积极作用,它的抑制作用会阻碍DDR,并使细胞对电离辐射敏感,从而诱导细胞死亡。该抗体与AlexaFluor®488偶联。AlexaFluor®488是一种流行的鲜绿色荧光染料,具有高pH稳定性。
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