Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
ThisantibodyisshippedwithitsantigenFREEofcharge!
- Peptide(C)HLVEVVPNGDLVRRD,correspondingtoaminoacidresidues201-215ofratCALCR(Accession P32214).
- Westernblotanalysisofratbrainmembrane(lanes1and3)andmousebrainlysate(lanes2and4):1,2. Anti-CalcitoninReceptor(extracellular) Antibody(#ACR-053),(1:200).
3,4.Anti-CalcitoninReceptor(extracellular)Antibody,preincubatedwiththenegativecontrolantigen.
- ExpressionofCALCRinratPC12cellsCellsurfacedetectionof CALCRinintactlivingratpheochromocytoma(PC12)cells.A,C.Extracellularstainingofcellsusing Anti-CalcitoninReceptor(extracellular) Antibody,(#ACR-053),(1:50).B,D.MergedimagesofAandC,eachwithitsrespectiveliveviewofthecells.
- 1.Segovia-Silvestre,T.etal.(2011)BMCRes.Notes4,407.
- 2.Barwell,J.etal.(2012)Br.J.Pharmacol.166,51.
- 3.Taboulet,J.etal.(1998)Hum.Mol.Genet.7,2129.
- 4.Gorn,A.H.etal.(1995)J.Clin.Invest.95,2680.
- 5.Gorn,A.H.etal.(1992)J.Clin.Invest.90,1726.
- 6.Gooi,J.H.etal.(2014)J.Endocrinol.221,181.
thecalcitoninreceptor(CTR)isaclassB G-proteincoupledreceptor (GPCR)thatsignalsviaactivationofcAMPandproteinkinaseC(PKC).Itisoneof15humanclassBGPCRs1,2.ApartfromCTR,calcitoninreceptor-likereceptor(CTLR)istheonlyotherB-classGPCRtobindcalcitonin.Todate,therearethreeknownhumanCTRmRNAisoformsarisingfromalternativesplicing3.
Calcitoninitselfisapeptidehormonethatcontributestobonecalcificationbydirectinhibitionofosteoclastswhichresorbthebonytissue.Itispartofasix-memberfamilythathasvariedfunctionalities.Bybindingtoreceptoractivitymodifyingproteins(RAMPs),CTRandCTLRcanformreceptorstoeverymemberofthecalcitoninfamily.Forinstance,RAMP1andCTRconstructthecalcitoningene-relatedpeptide(CGRP)receptor.
BothCTRandCTLRhaveseventransmembranedomains,anintracellularC-terminal,andanextracellularN-terminal.Toactivatethereceptors,theirN-terminalmustfirstcapturetheC-terminaloftheligandandthencarriedtothetransmembraneloopswhereitbestowsaconformationalchangeuponthem.
Besidesthebone,CTRhasprominentrolesinthekidney,CNS,placenta,ovaries,sperm,lung,andinthebrain4,5.
Inosteoclasts,theexpressionofCTRsdeclineswithage.Interestingly,polymorphismoftheCTRgeneisstronglyassociatedwithdecreasedfracture-riskinpost-menopausalwomen3,6.
AlomoneLabsispleasedtoofferahighlyspecificantibodydirectedagainstanepitopeofratCT-R.Anti-CalcitoninReceptorAntibody(#ACR-053)canbeusedinwesternblotandlivecellimagingapplications.IthasbeendesignedtorecognizeCT-Rfromhuman,ratandmousesamples.