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蚂蚁淘在线 / 品牌中心 / DiscoverX / DiscoverX/PathHunter® Tocilizumab Bioassay Kit/200dp(2x96-well)/93-1045B3-00109
商品描述
The PathHunter Tocilizumab Bioassay is an application of the DiscoverX Dimerization Assay platform. The assay is designed to detect the ligand-induced interaction of the interleukin-6 receptor (IL-6R) with the interleukin-6 signal transducer protein (IL-6ST). The cells have been engineered to co-express IL-6ST fused to PK, and IL-6R fused to EA. Binding of an agonist to the IL-6R causes multimerization of the IL-6R and IL-6ST receptors, resulting in activation of downstream signaling events. This brings the two b-gal fragments into close proximity, forcing complementation. Heterodimerization of the two receptor chains results in the formation of a functional b-gal enzyme that hydrolyzes the substrate to generate a chemiluminescent signal. Blocking IL-6 binding with an anti-ligand or anti-receptor antibody can prevent this interaction, resulting in a loss of signal.The bioassay kit contains all reagents needed for the assay, including pre-qualified cryopreserved cells, detection reagents, cell plating reagent, positive control agonist, and assay plates.
- Target/Description
- interleukin 6 receptor
- Group
- Interleukin Receptor
- Cell Background
- U2OS
- Species
- Human
- Accession Number
- NM_000565.3
- Agonist/Ligand
- IL-6
- Assay Measures
- Dimerization
- Bioassay Target Name
- IL6R/IL6ST
- Assay Qualified With
- Actemra
- Drug Trademark Owner
- Actemra is a registered trademark of Chugai Seiyaku Kabushiki Kaisha Corp., a member of the Roche Group.
How it works
DiscoverX化学发光检测产品
产品
猫号
兼容细胞系
PathHunter检测套件
93-0001系列
PathHunterβ-抑制蛋白
PathHunter内部化
PathHunter药物贩运
PathHunter受体酪氨酸激酶
PathHunter胞质酪氨酸激酶
PathHunter通路
PathHunter核激素
PathHunter父母
PathHunter闪光检测套件
93-0247系列
PathHunter二聚化
PathHunter药物贩运
适用于生物制剂的HitHunter®cAMP测定
90-0075LM系列
cAMP Hunter Gs / Gi偶联细胞系
小分子的HitHunter cAMP测定
90-0075SM系列
KILR®检测试剂盒
97-0001系列
KILR靶细胞系/池
PathHunter生物测定检测试剂盒
93-0933系列
PathHunter生物测定
PathHunter生物测定ED检测试剂盒
93-1043系列
InCELL检测试剂盒
96-0079系列
InCELL Hunter细胞系
产品变更通知
HitHunter cGMP分析
90-0039系列
PathHunter NPR1功能测定
DiscoverX用于EFC组件表达功能确认的检测试剂盒
产品
猫号
有可能的使用
EFC BlocDetect™套件
92-0004
通过鉴定抑制β-半乳糖苷酶互补或β-半乳糖苷酶活性的化合物来检测假阳性结果
ProLink检测套件
92-0006系列
在表达ProLink载体的亲代细胞中检测ProLink标记的蛋白
PathHunter ProLabel检测试剂盒
93-0180系列
在表达ProLabel载体的亲代细胞中检测ProLabel标记的蛋白
PathHunter EA检测套件
93-0183系列
在表达EA的亲代细胞(例如ENDO-EA,MEM-EA,NUC-EA,β-arrestinEA和SH2-EA)中检测β-半乳糖苷酶(酶受体,EA)的大蛋白片段
PathHunter PL / PK检测试剂盒
93-0812系列
用于使用PathHunter Pathway细胞系进行EFC组件的功能确认
DiscoverX荧光检测产品
产品
猫号
有可能的使用
HitHunter钙免洗PLUS检测
90-0091系列
使用天然钙Gq耦合细胞系监测GPCR和离子通道激活
腔肠素h
90-0084
ADP Hunter™Plus
90-0083系列
通过监测ATP消耗来检测激酶活性
ADP Quest™
90-0071系列
用于动力学或终点分析的激酶和抑制剂表征