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蚂蚁淘在线 / 品牌中心 / DiscoverX / DiscoverX/PathHunter® PD-1 (SHP1) Signaling Bioassay Kit/1000dp(10x96-well)/93-1104Y19-00118
商品描述
The PathHunter PD1 Signaling Bioassay consists of human cells engineered to stably express an ED-tagged immune checkpoint receptor, PD-1, while EA is fused to either the full length or the phosphotyrosine-binding SH2 domain of an intracellular signaling protein, SHP1. Engagement of the receptor by its ligand, or agonist antibodies results in phosphorylation of the receptor’s cytosolic tail. The SH2-domain fused to EA binds the phosphorylated receptor, forcing complementation of ED and EA, resulting in formation of an active β-gal enzyme, which hydrolyzes the substrate to generate a chemiluminescent signal.The bioassay kit contains all reagents needed for the assay including pre-qualified cryopreserved cells, detection reagents, cell plating reagent, positive control agonist, and assay plates.
- Target/Description
- Programmed cell death protein 1
- Group
- T cell receptor (inhibitory)
- SH2 Domain
- SHP1
- Cell Background
- Jurkat
- Species
- Human
- Accession Number
- NM_005018.2
- Agonist/Ligand
- Anti-PD1 Antibody [NAT105]
- Antagonist/Inhibitor
- Anti-PD1 antibody [NAT105]
- Assay Measures
- SH2 Recruitment
- Bioassay Target Name
- PD-1
- Assay Qualified With
- Keytruda®
- Drug Trademark Owner
- Keytruda®is a registered trademark of Merck & Co., Inc.
How it works
