VirusDextramerCollection1-APC

ProductCode:RX03-APC

Collectionof4carefullyselectedvirus(CMV,EBV,BKV&Influenza)specificMHCIDextramers(+1control),forsensitiveandspecificdetectionofantigen-specificTcellsusingflowcytometry.EachmulitmerisAPClabeled.
Amount:5x50tests
Application(s):Cellularimmuneresponse,Immunemonitoring,T-cellassays
Indication(s)/Topic(s):Control,Vaccination,Infection,Transplantation
DeliveryTime:10-12days

MHCIDextramerCollections
MHCIDextramerscombineadextranpolymerbackbonewithahighnumberofMHCandFluorochromemoleculesprovidingmulitplebindingdomainsforantigen-specificTcellreceptorbinding.Thisenhancestheirstainingintensityandincreasesresolutionaswellassignal-to-noiseratiowithminimalbackgroundstaining.Inaddition,thedextranbackbonestABIlizestheMHC-peptidecomplexesandfluorochromes.ThevirussetisusefulforenumerationofCMV-,EBV-,Influenza-,andBKvirus-specificCD8+Tcells,e.g.forimmunemonitoringinimmune-compromisedindividualsfollowingtransplantation,testingofvirus-specificvaccinesorprofilingofvirus-specificTcellresponses.TheDextramersareprovidedasAPCconjugates.

Thefollowing5DextramersareprovidedwithAPClabel

Dextramer:HLA-A*0201/NLVPMVATV-Virus:CMV
Dextramer:HLA-A*0201/GLCTLVAML-Virus:EBV
Dextramer:HLA-A*0201/GILGFVFTL-Virus:Influenza
Dextramer:HLA-A*0201/LLMWEAVTV-Virus:BKvirus
Dextramer:HLA-A*0201/NegativeControl

BenefitsofMHCIDextramers®
-MoreMHCmoleculesandmorefluorochromesthanconventionalMHCmultimers
-Highersensitivity(importantforlow-affinityepitopesascommonlyfoundincancer&autoimmunedisease)
-Highstability
-Custom-madeDextramersavailableuponrequest

VirusDextramerCollection1-APC

SelectedReferencesforMHCIDextramers®
"CombinationImmunotherapyafterASCTforMultipleMyelomaUsingMAGE-A3/Poly-ICLCImmunizationsFollowedbyAdoptiveTransferofVaccine-PrimedandCostimulatedAutologousTCells"
Rapoportetal.,ClinCancerRes(2014)-PMID:24520093
"MolecularImprintofExposuretoNaturallyOccurringGeneticVariantsofHumanCytomegalovirusontheTcellRepertoire"
Smithetal.,SciRep(2014)-PMID:24509977
"ProbingtheeffectorandsuppressivefunctionsofhumanTcellsubsetsusingantigen-specificengineeredTcellreceptors"
Wanetal.,PLoSOne(2013)Fulltext
"HumanNaturalKillerCellsPreventInfectiousMononucleosisFeaturesbyTargetingLyticEpstein-BarrVirusInfection"
Chijiokeetal.,CellRep(2013)-PMID:24360958