TheXPMediaandCloneMediaforMouseHybridomaGenerationisacompletesolutionthatsupportsallstagesofhybridomacelllinedevelopmentfromfusiontoscaleup.Optimizedtosupporttheselectionandgrowthofhybridomaclones usingtheMolecularDevicesClonePixsystems,thekitisalsocompatIBLewithotherappropriatemethods.
KeyAdvantages:- Thesemi-solidCloneMediamethodofcloningpreventstheovergrowthoffast-growingclones,allowingfortheselectionofpotentiallyvaluableslow-growingclones
- Hands-ontimecanbereducedwhentheworkflowiscombinedwithaClonePixsystem. Hypoxanthine-aminopterin-thymidine(HAT)selectionandcloningofhybridomasareaccomplishedinonestep,minimizingtherequiredtimeandmaterials
OurXPMediaandCloneMediaCompleteKitforMouseHybridomaGeneration(P/NK8861)iscomposedofthefollowingcomponents.Allcomponentscanbesoldseparately.
Component | Description |
---|---|
XPMediaPre-FusionMyelomaGrowthMediumandHybridomaExpansionMedium(withoutHT)P/NK8862 | Supportsthegrowthofmyelomacellsbeforefusion.Alsosupportsexpansionofhybridomaclones.Doesnotcontainhypoxanthineorthymidine(HT). |
XPMediaHybridomaFusionMediumP/NK8863 | Usedtowashcellsbeforefusionandduringfusionprocess.Doesnotcontainsupplementstosupportgrowth. |
XPMediaHybridomaFusionRecoveryMediumP/NK8864 | UsedtopromotehybridomaviABIlityafterthefusionprocessbutbeforecloneselection.Doesnotcontainhypoxanthine,aminopterin,andthymidine(HAT). |
CloneMediaHybridomaSemi-SolidSelectionandCloningMedium(withHAT)P/NK8865 | Usedafterfusionofsplenocytesandmyelomacellstoselectandclonehybridomasinonestep.Methycellulose-basedformulaoptimizedforcolonyformation.Equallysuitableforfreshfusionsandforstablehybridomacelllines. |
XPMediaHybridomaGrowthMedium(withHT)P/NK8866 | Optimizedforhybridomaexpansionfollowingcloneselectionandcolonypicking.Containshypoxanthineandthymidine(HT)andisusedtoweanhybridomasoffaminopterinfromtheselectionprocess. |
HybridomaPolyethyleneGlycol(PEG)forCellFusionP/NK8868 | Usedforthefusionofmousesplenocytesandparentalmyelomacellstogeneratehybridomas.PEGispresentasa50%solutioninDMEM. |