RNasestandsforRibonucleasewhichisaRNAdigestingenzyme.RNaseconsistsof124aminoacidsinonepolypeptidechainwith4disulphidelinkages. Ribonucleasescanbedividedinto Endoribonucleases and Exoribonucleases. RNaseshaveimportantrolesinRNAdegradationandturnoverinallorganisms. RibonucleaseenzymecanunwindtheRNAhelixbycomplexingwithsingle-strandedRNA.RNaseAisanendoribonucleasewithfunctionsinRNAmetabolismandregulationofgeneexpression. Itwasfoundtoplayrolesindiseasessuchasautoimmunediseases,renalinsufficienciesandpancreasdisorder. Morerecently,anantitumoractivitywasalsoreportedforanRNaseAwithcytotoxicandcytostaticproperties. ThemembersofRNaseAsuperfamilyarewidelyexpressedandpresentinserumandtissuesofmammals. Theribonucleolyticactivityinthepancreasofruminantsisparticularlyhigh,perhapstodigestthelargeamountofRNAproducedbystomachmicroorganisms.
Applications:
TheenzymecanbeusedtohydrolyzeRNAfromproteinsamples.ItiscompatIBLeforuseinRNaseprotectionassays,toremoveunspecificallyboundRNA,intheanalysisofRNAsequences,tohydrolyzeRNAcontainedinproteinsamples,andinthepurificationofDNA.
AdditionalInformation
| Synonyms | bovinepancreaticribonucleaseA,RNaseAEC3.1.27.5 |
|---|---|
| Product# | R-2000 |
| CAS# | 9001-99-4 |
| ChemicalName | RIBONUCLEATE3"-PYRIMIDINOOLIGONUCLEOTIDOHYDROLASE |
| MDLNumber | MFCD00132181 |
|---|---|
| ChemACX | X1037961-6 |
