Protease K; Endopeptidase K; Tritirachium alkaline proteinase

P-1264

29.3 kDa

White to off-white lyophilized solid

≥99%

50mM Tris-HCl (pH 7.5), 3mM CaCl2, 50% Glycerol

4°C, desiccated

Not Available

This PCR Grade Proteinase K is particularly suitable for isolating nucleic acids for amplification reactions, as well as for the analysis of membrane structures by modifying proteins and glycoproteins on cell surfaces. It is extremely effective on native proteins and can therefore be used to rapidly inactivate endogenous nucleases such as RNases and DNases during nucleic acid isolation. This property makes Proteinase K particularly suitable for the isolation of native RNA and DNA from tissues or cell lines. It promotes cell lysis by activating a bacterial autolytic factor. Because the enzyme is tested for the absence of RNases and DNases, and is virtually free of DNA, it is especially suitable for isolating PCR and RT-PCR templates. Proteinase K can also be used to remove cellular debris during the preparation of colony lifts, and to treat tissue sections to ensure efficient probe infiltration during in situ hybridization.

Wear personal protective equipment. Ensure adequate ventilation. Do not get in eyes, on skin, or on clothing. Avoid ingestion and inhalation. Avoid dust formation. Wash hands before breaks and immediately after handling the product.

P-1264, G1340A.pdf

P-1264, G1340.pdf