Enzolifesciences/GluR1 (extracellular) polyclonal antibody/BMLSA6120050/50µl相关交流-蚂蚁淘在线

Enzolifesciences/GluR1 (extracellular) polyclonal antibody/BMLSA6120050/50µl

mayitao /发布时间:1631122203 /浏览量:63

ProductSpecification:

AlternativeName:	Glutamatereceptor1,AMPA-selectiveglutamatereceptor1,GlutamatereceptorionontropicAMPA1

Host:	Rabbit

Immunogen:	Syntheticpeptidecorrespondingtoaa271-285ofratGluR1(GRIA1).

UniProtID:	P19490

Speciesreactivity:	Mouse,Rat

Applications:	IHC,WB

RecommendedDilutions/Conditions:	Immunohistochemistry(1:100) WesternBlot(1:200) Suggesteddilutions/conditionsmaynotbeavailableforallapplications. Optimalconditionsmustbedeterminedindividuallyforeachapplication.

BlockingPeptide:	Included:GluR1(extracellular)ControlPeptide(100µg).Suppliedlyophilized.

PurityDetail:	Affinitypurified

Formulation:	LyophilizedfromPBS,pH7.4,1%BSAand0.025%sodiumazide.

Reconstitution:	Tolyophilizedantibody,add50µlor200µl(dependingonwhichsizewaspurchased)ofdeionizedwater.Furtherdilutionsshouldbemadeusingacarrierproteinsuchas1%BSA.Tolyophilizedcontrolpeptide,add100µlofdistilledwater.

Use/Stability:	Lyophilizedantibodyandlyophilizedpeptidecanbestoredintactatroomtemperatureforseveralweeks.Forlongerperiodstheyshouldbestoredat-20°C.Afterreconstitution,theantibodysolutioncanbestoredat4°Cforupto2weeks.Forlongerperiods,smallaliquotsshouldbestoredat-20°Corbelow.Centrifugeallantibodypreparationsbeforeuse(10,000Xgfor5minutes).Afterreconstitution,controlpeptideshouldbestoredat-20°C.

Shipping:	ShippedonBlueIce

LongTermStorage:	-20°C

ScientificBackground:	GluRreceptorsarepermeabletocationsNaandKandCa2+.GatingofGlureceptorsbyglutamateisextremelyfastandthereforetheGlureceptorsmediatemostexcitatory(depolarizing)currentsinthebrainduringbasalneuronalactivity.

TechnicalInfo/ProductNotes:	PREADSORPTIONCONTROL:1µgcontrolpeptideper1µgantibody.Prepare2tubes.Inthefirst,dilutethenecessaryamountofantibodyin200-500µlofPBS,1%BSAand0.025%sodiumazide.Preparethesecondtubeidenticallytothefirstwiththeadditionoftheappropriateamountofpeptide.Incubateatroomtemperaturefor1hr.Centrifugebothtubesat10,000Xgfor5min.Usethesupernatantsfromtubes1and2forparallelexperiments.

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Enzolifesciences/GluR1 (extracellular) polyclonal antibody/BMLSA6120050/50&micro;l

ProductSpecification:

Enzolifesciences/GluR1 (extracellular) polyclonal antibody/BMLSA6120050/50µl