- Cellulaseassays资讯
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Cellulase assays
Cellulaseenzymesshowactivityduringtheripeningofsomefruits,wheretheireffectsoncellwallsresultsinsofteningofthefruit.Incasesofprogrammedcelldeath,suchastheformationofaerenchyma(largeairspacesinthecortexofplantsinfloodedsoils),andintheabscissionzonesofleavesandfruits,cellulasesareonceagainveryactive,breakingdownthecellulosewallsofthedeadcells.TwopossIBLeprotocolsaredescribedhere,ageldiffusionassaymethodandaviscosityreductionassaymethod. Geldiffusionmethod Afruitextractisplacedinawellinsomeagarcontainingcarboxymethylcellulose(CMC),and,ascellulasemoleculesdiffuseoutwardsfromthewell,theydestroycellulosemolecules.Themoreconcentratedtheenzymesinthewell,thelargerthezoneofcellulosedestruction. Wellscontainingcellulaseshouldhaveaclearzonearoundthem,andthediameterofthezonegivesameasureofthecellulaseactivityinthatwell. Viscosityreductionmethod Thetechniqueisbasedontheactionofcellulaseenzymeswhichshortenthelengthsofcellulosemoleculesinaviscoussolutionofwallpaperpasteandcauseittobecomelessviscous(runnier). Themoreactivetheenzyme,thegreaterthereductioninviscosity,andsotheshorterthedrainagetimes.
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