The YEAST MEDIA相关交流-蚂蚁淘在线

The YEAST MEDIA

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COMPLEXMEDIA

YPADmediumorAlsoCalledYPDplusAdenineYeastExtract-Peptone-DextroseplusAdeninemedium

6.0gmyeastextract(Difco)12.0gmPeptone(Difco)12.0gmGlucose60mgadeninehemisulphate600mldistilledwaterBacto-agar(Difco)10g(addedforagarmedium)

Sterilisebyautoclavingat121°Cfor15min.

Wealwaysusea1literflaskandmake600mlsofmediabecauseitgivesexactly20plates(1Sleeveofplates).Inadditionthisvolumeofliquidismoreeasilyhandledwhenpouringafterautoclaving.

SYNTHETICCOMPLETEDROPOUT(SCdrop-out)MEDIUM

4.0gmDifcoYeastNitrogenBase(w/oaminoacids)4.0gm12.0gmglucose0.50gmSyntheticCompleteDropOutMix600mldistilledwater10.0gmDifcoBactoAgar(addforsolidmedium)

Addtheingredients(exceptAgar)tothewaterandmixandadjustthepHto5.6with10NNaOH.(THISSTEPISIMPORTANTFORhighestefficiencyTRANSFORMATION)Autoclaveat121°Cfor15min.

SyntheticCompletedrop-outMediumMix(SCdrop-out)

SupplementAmountNotethehintaboutmakingthismediabelow

2.0gmAdeninehemisulfate*2.0gmArginineHCl2.0gmHistidineHCl*2.0gmIsoleucine2.0gmLeucine*2.0gmLysineHCl2.0gmMethionine3.0gmPhenylalanine6.0gmHomoserine3.0gmTryptophan*2.0gmTyrosine1.2gmUracil*9.0gmValine

Omittheappropriatecomponent(s),indicatedinboldtype,toprepareSC-Ade,SC-His,SC-Leu,SC-Trp,SC-Trp/Leu,andSC-Trp/Leu/His.Combinetheingredientsinaclean250mlplasticbottle.Addthreeorfourcleanglassmarblesandshakevigorouslytomix.Thisquantitywillbesufficientforapproximately100(600ml)batchesofSCdrop-outmedium.Somerecipescallfor20aminoacidsintheSCmixturehowevermostyeaststrainsdonotneedallofthem.Inadditionsomerecipesdonotusehomoserineduetotheexpense,itcanbereplacedbytheadditionofserineandthreonine(2gramseach).

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The&nbsp;YEAST&nbsp;MEDIA

The YEAST MEDIA