产品概述

FITCConjugation Kit / FITC Labeling Kit ab102884 uses a simple and quick process for FITC labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides.Learn about ourantibody labeling kits and their advantages.

We recommend FITC Conjugation Kitab188285 as an alternative this kit using a faster, newer protocol.

To conjugate an antibody to FITCusing this kit:
- add modifier to antibody and incubate for 3 hrs
- add quencher and incubate for 30 mins
The FITC conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.

Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapidantibody purification and concentration kits. Use theFAQto learn more about the technology, or about conjugating other proteins and peptides to FITC.

Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.

This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link Fluorescein Labeling Kit. 707-0005 is the same as the 100 g size. 707-0010 is the same as the 3 x 100 ug size. 707-0030 is the same as the 3 x 10 ug size. 707-0015 is the same as the 1 mg size.

Amount and volume of antibody for conjugation to FITC

1 Using the maximum amount of antibody may result in less labelling per antibody.

2Idealantibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies 2 mg/ml or Buffer Requirements for Conjugation

Buffer should bepH 6.5-8.5.

Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.

1Tris buffered saline is almost always 50 mM / 0.6%

Incompatible buffer constituents

If a constituent of the buffer containing your antibody or protein is not listed above, please check theFAQorcontact us.

Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acidsare not present: antibodies in ascites fluid, serum or hybridoma culture.

Storing and handling conjugation kits

Lyophilized Lightning-Link components are hygroscopic.

Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.

Immunohistochemistry (PFA perfusion fixed frozen sections) - FITC Conjugation Kit- Lightning-Link (ab102884)Image from Wills, Jonathan, et al., PLoS One, 6(3): e17953; doi: 10.1371/journal.pone.0017953. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

Wills, Jonathan, et al used FITC Conjugation Kit - Lightning-Link (ab102884) as part of examining the aggregation and co-localization of -Syn with pGSK-3 and p-Tau in the striatum of A53T -Syn mutant mice (A53T Tg) and wild-type mice (WT). They used the kit to conjugate FITC to Anti-p-GSK-3 (pY216) antibody for use in immunohistochemistry (PFA perfusion fixed frozen sections).
Right panels constitute merged image of left panels. Sections of striatum of A53T Tg and age-matched WT mice were stained with anti- -Syn antibody conjugated to Texas Red (red) and anti-p-GSK3 conjugated to FITC (green). Nuclei were stained with DAPI (blue).

Immunocytochemistry - FITC Conjugation Kit- Lightning-LinkImage from Lim, Tony KY, et al., J Neurosci., 35(8):3346-59, doi: 10.1523/JNEUROSCI.4040-14.2015. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

Lim, Tony KY, et al used FITC Conjugation Kit - Lightning-Link (ab102884) as part of examining traumatic nerve injury. They used the kit to conjugate FITC to mouse anti-neurofilament 160/200 for use in immunohistochemistry.B, Contralateral nerves were stained for hypoxyprobe-1, DAPI, and neurofilament 160/200 (NF-M/L, axons). E, Ipsilateral nerves were subjected to the same staining. The area in proximity to the injury site is shown. Hypoxia, as shown by hypoxyprobe-1 staining, is observed in a region close to the injury site, in which axons, macrophages, and Schwann cells are all found to be hypoxic. This hypoxic region, in which nerve fibers were ligated, is delineated by the white dotted line. Areas outside of the dotted regions containing nonligated nerve fibers still have more hypoxyprobe deposition than contralateral nerves.

Flow Cytometry - FITC Conjugation Kit- Lightning-LinkImage from Weagel, Evita G., et al., Cancer cell international 18.1 (2018): 1-14. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

Weagel, Evita G., et al used FITC Conjugation Kit - Lightning-Link (ab102884) as part of examining thymidine kinase 1 in lung, breast, and colorectal malignancies. They used the kit to conjugate FITC to three custom and a commercial anti-human thymidine Kinase 1 (TK1) antibodies for use in flow cytometry.
Membrane TK1 expression in of colon, breast, and lung cancer cell lines. Flow cytometry analysis of cell lines treated with anti-TK1 antibodies. a Quantification of TK1 expression on the cell membrane of HT-29 and SW620 cell lines stained with FITC or APC-conjugated anti-TK1 antibodies. b Quantification of TK1 expression on the cell membrane of MCF7 and MDA-MB-231 cell lines. The top bar graph shows MCF7 and MDA-MB-231 cell lines stained with FITC or APC-conjugated anti-TK1 antibodies. c Quantification of TK1 expression on the cell membrane of NCI-H460 and A549 cell lines stained with FITC or APC-conjugated anti-TK1 antibodies. Statistical analysis was performed by comparing the mouse isotype control fluorescent levels to those of A72, A74, CB1, or ab91651. *P 0.05; **P 0.005; ***P 0.001; ns = P 0.05

This illustration demonstrates a general procedure and will slightly vary dependent on the conjugate used.

发表研究结果有使用 ab102884？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab102884 被引用在 42 文献中.

Huang KY et al. Humanized COVID-19 decoy antibody effectively blocks viral entry and prevents SARS-CoV-2 infection. EMBO Mol Med 13:e12828 (2021). PubMed: 33159417 Micheva-Viteva SN et al. Increased Mortality in Mice following Immunoprophylaxis Therapy with High Dosage of Nicotinamide in Burkholderia Persistent Infections. Infect Immun 87:N/A (2019). PubMed: 30323029 Liu G et al. Fibulin-1c regulates transforming growth factor-ß activation in pulmonary tissue fibrosis. JCI Insight 5:N/A (2019). PubMed: 31343988 Dumigan A et al. A Porcine Ex Vivo Lung Perfusion Model To Investigate Bacterial Pathogenesis. mBio 10:N/A (2019). PubMed: 31796543 Zhdanov DD et al. Murine regulatory T cells induce death of effector T, B, and NK lymphocytes through a contact-independent mechanism involving telomerase suppression and telomere-associated senescence. Cell Immunol 331:146-160 (2018). PubMed: 29935763 View all Publications for this product
We conjugated FITC to BSA protein. Then, the protein was bound to our microparticles for viewing. The left image is a brightfield image of the microparticles and the right image is the microparticles with BSA-FITC conjugate bound to them.
The kit was easy enough for me to hand this off to an undergraduate, albeit with some explanation of the directions. These were a tad bit confusing at first, and not really clear on the volume of antibody to add (they give you a couple options since the Ab concentration of course matters, but didn't make it clear they were different options).
Otherwise the kit work fantastically with some store bought, pure, concentrated antibody! It actually gave better results than our previous method of using a conjugated secondary antibody when used in Flow Cytometry after doing an Intracellular Stain.

There is no difference in sensitivity between the rapid (eg. ab188285) and the standard (eg. ab102884) labeling kits. The only difference between the two is the incubation time required for the reaction.

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This antibody will detect the whole cells, and because the antiserum has not been absorbed (only Protein A purified) it may react with related microorganisms.


As this antibody is suitable for conjugation purpose according to the datasheet, I would like to sduggest your customer is having a look at our FITC conjugation kit.

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Thank you for your enquiry.

The EasyLink conjugation kits are designed so that they can be used to label proteins other than antibodies. In this case you should be able to label the Fc fused protein in the same way as labeling an antibody. However as the size of their protein is likely different to that of an average antibody, please find advice we give in these situations below:

As the protocols provided were optimised for labeling IgGs, we would recommend to adjust the amount of material added to the label vial to allow for molecular weight difference. This should be done without changing the volume added to the vial, as this could affect the conjugation efficiency. As a rough guideline, we would recommend changing the amount of material proportionally to the size difference with IgGs. An average IgG is about 160kD, therefore for a target that is the size of an antibody (about 80kD), add as much to the vial.

Finally, please note the usual EasyLink requirements will still apply: the target should be purified, in a suitable amine free buffer and not contain any additives such as Azide, BSA, Tris or Glycine.

I hope that this helps. If you have any additional questions please do not hesitate to contact me.

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Thank you for contacting us.

The labeling kit works by targeting free amines groups on your antibody. We therefore cannot guarantee that the label will not bind to the FAB area, however this would be the case for any other amine orientated labeling technology. Although this might seem like an issue , it does not mean that the FAB will become unreactive, and we see successful conjugations in the great majority of cases.

The amine binding would only become an issue if there is an amine group within the antibody binding site itself.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote RABMAB-XBSMG in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource rid=15447

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Thank you for your inquiry and your interest in our products.

I will have to double-check with the lab regarding glycosylation of the antibodies since I do not have this information readily at hand. I will let you know once I have received this information.

For the EasyLink kits, there is a quenching step which removes any excess label and reduces background. You can find more information about the labeling process here: https://www.abcam.com/index.html?pageconfig=resource rid=13148 source=pagetrap viapagetrap=easylink

Additional FAQs regarding these kits can be found here: https://www.abcam.com/index.html?pageconfig=resource rid=13156

I will email you a quote for the 4 products. Currently we are running a promotion where if you buy any primary, you can get a RabMAb for free! Just mention the code RABMAB-XBSMG on the order and the cost of the rabbit monoclonal antibody will be removed from the order.

I hope this information helps. I will be in touch again with more information regarding the antibodies you requested.

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Thank you for your interest in the FITC conjugation kit. Unfortunately, it will be difficult to achieve a consistent ratio of FITC : IgG without extensive testing of the kit to determine the appropriate conditions. We have not done this testing, as the kit was developed for ease of producing a conjugate that simply gives a bright signal, rather than producing a conjugate of a known ratio. Further, the components of the reagents are considered proprietary, which makes knowing where to start difficult. To do what you want, I think it will make more sense to work with a standard FITC conjugation protocol, using known reagents and quantities, and testing various combination of FITC and antibody.

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Thank you for contacting us.

The fluorescein in ourKit is not simply fluorescein and it is not FITC either. Our fluorescein has been specially treated with proprietary Easy-Link chemistry to make the unique one-step conjugation possible.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
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Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES
For licensing inquiries, please contact partnerships@abcam.com