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Rabbit Papillomavirus type 16 L1capsids IgG antibody ELISA Kit
货号: | AE62701RB |
样本: | Serum,Plasma,OtherBIOLOGicalfluids |
适应物种: | Rabbit(Oryctolaguscuniculus) |
应用: | Qualitative |
检测方法: | Sandwich |
检测限: | RequestInformation |
供应商: | 华夏远洋 |
数量: | 999 |
规格: | 96T/48T |
别名:N/A
检测范围:RequestInformation
产品概述:
特异性:ThisassayhashighsensitivityandexcellentspecificityfordetectionofRabbitIgG.Nosignificantcross-reactivityorinterferencebetweenRabbitIgGandanalogueswasobserved.
回收率:MatriceslistedbelowwerespikedwithcertainlevelofrecombinantRabbitIgGandtherecoveryrateswerecalculatedbycomparingthemeasuredvaluetotheexpectedamountofRabbitIgGinsamples.
线性:ThelinearityofthekitwasassayedbytestingsamplesspikedwithappropriateconcentrationofRabbitIgGandtheirserialdilutions.Theresultsweredemonstratedbythepercentageofcalculatedconcentrationtotheexpected.
稳定性:ThestABIlityofELISAkitisdeterminedbythelossrateofactivity.Thelossrateofthiskitislessthan5%withintheexpirationdateunderappropriatestoragecondition.Thelossratewasdeterminedbyacceleratedthermaldegradationtest.Keepthekitat37°Cfor4and7days,andcompareO.D.valuesofthekitkeptat37°Cwiththatofatrecommendedtemperature.(referringfromChinaBiologicalProductsStandard,whichwascalculatedbytheArrheniusequation.ForELISAkit,4daysstorageat37°Ccanbeconsideredas6monthsat2-8°C,whichmeans7daysat37°Cequaling12monthsat2-8°C).
测试原理:Thisassayemploysatwo-sitesandwichELISAtoquantitateIgGinsamples.AnantibodyspecificforIgGhasbeenpre-coatedontoamicroplate.StandardsandsamplesarePipettedintothewellsandanyIgGpresentisboundbytheimmobilizedantibody.Afterremovinganyunboundsubstances,abiotin-conjugatedantibodyspecificforIgGisaddedtothewells.Afterwashing,StreptavidinconjugatedHorserADIshPeroxidase(HRP)isaddedtothewells.Followingawashtoremoveanyunboundavidin-enzymereagent,asubstratesolutionisaddedtothewellsandcolordevelopsinproportiontotheamountofIgGboundintheinitialstep.Thecolordevelopmentisstoppedandtheintensityofthecolorismeasured.
温馨提示:不可用于临床治疗。
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