标签抗体

flag标签抗体偶联affinity gel亲和凝胶

货号:AT0078
抗体名:flag标签抗体偶联affinitygel亲和凝胶
抗体英文名:antiflagaffinitygel
靶点:flag
应用范围:IP,Co-IP
标记物:agarosebeads
克隆性:
保存条件:–20°C
形态:液体
规格:1ml,10ml

可来电或是QQ索取详细产品说明书

折扣信息请登录上海维景公司网站  http://www.weijingbiotech.com/cmctag/affinity_gel.html

 

AgarosebeadsConjugatedanti-FLAGMousemonoclonalantibody

CatalognumberAT0078      Size1ml

 

Specificity Anti-FLAGAgarosebeads(Affinitygel)isamousemonoclonalantibodythatiscovalentlyattachedtoagarosebeadsbyhydrazidelinkage.TheantibodybindsFLAGattheN-terminal,Met-N-terminal,C-terminalandinternallocationsoffusionproteins.

Testedapplications:  Immunoprecipitation(IP),

40mlofgelsuspension(~20μlofpackedbeads)for~1000μgofcrudetotalproteinsolution.

Optimaldilutions/concentrationsshouldbedeterminedbytheenduser.

Reactivity:  TheantibodydetectsandcapturesDYKDDDDK-TaggedfusionproteinsoverexpressedinmammaliancellsorE.coli.

Immunogen:  Thismonoclonalantibodyisproducedbyimmunizingmouseswithasyntheticpeptide(KLH-coupled)containingDYKDDDDK.

Form:  Theproductissuppliedasa50%suspensionin50%glycerolwith10mMsodiumphosphateand150mMsodiumchloride,pH7.4,containing0.02%(w/v)sodiumazide

Binding capacity: >50nmolesofFLAGfusionproteinper1mlofsettledgel.

Isotype:  MouseIgG1

RecommendedIPLysisbufferformammaliancells: 50mMTrisHCl,pH7.4,with150mMNaCl,1mMEDTA,and1%TRITONX-100.

Storageinstruction:

Thegelissuppliedin50%glycerol,sothegelcanbestoredat–20°Cformaximumstability.Theunopenedproductisstableforoneyearwhenstoredasindicated.

 

Forusedandrecycledgel,thebeadsshouldbecleanedandstoredin50%glycerolwithTBSorPBSbuffercontaining0.02%sodiumazidetoprotecttheproduct.Storedat–20°Cformaximumstabilityor4°Cforshortterm(1-2weeks).Donotfreezeintheabsenceofglycerol.

 

 

产品简述:标签抗体偶联的agarosebeads又名标签抗体affinitygel,可无需ProteinA/Gbeads,直接抓取目的标签融合蛋白,主要用于对抗体亲和力和特异性要求极高的免疫沉淀实验(IP)、免疫共沉淀(Co-IP)、染色质免疫共沉淀实验(ChIP)。
 
货号标签抗体偶联亲和凝胶货期应用实验规格
AT0078Anti-Flag-Tag(AgarosebeadsConjugated)affinitygel现货IP,Co-IP1ml
AT0079Anti-HA-Tag(Agarosebeads Conjugated)affinitygel现货IP,Co-IP1ml
AT0080Anti-C-Myc-Tag(Agarosebeads Conjugated)affinitygel现货IP,Co-IP1ml
AT0084Anti-His-Tag(Agarosebeads Conjugated)affinitygel现货IP,Co-IP0.4ml
AT0082Anti-VSV-G-Tag(Agarosebeads Conjugated)affinitygel现货IP,Co-IP0.4ml
AT0083Anti-GFP(Agarosebeads Conjugated)affinitygel现货IP,Co-IP0.5ml
AT0081Anti-V5-Tag(Agarosebeads Conjugated)affinitygel现货IP,Co-IP2ml
AT0087Anti-S-Tag(Agarosebeads Conjugated)affinitygel现货IP,Co-IP0.4ml
AT0088Anti-T7-Tag(Agarosebeads Conjugated)affinitygel现货IP,Co-IP0.4ml
AT0086Anti-RFP(Agarosebeads Conjugated)affinitygel现货IP,Co-IP0.4ml


更多标签抗体偶联AgaroseBeads请浏览官网:http://www.cmctag.com/HotSpots/antibodiesconjugatedagarosebeads.html


 
温馨提示:不可用于临床治疗。

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