缺氧诱导因子1α 单克隆抗体相关交流-蚂蚁淘在线

MouseAnti-HIF-1Alpha antibody

产品编号	hzm-51085M
英文名称	HIF-1Alpha
中文名称	缺氧诱导因子1α单克隆抗体
别名	ARNTinteractingprotein;ARNT-interactingprotein;BasichelixloophelixPASproteinMOP1;Basic-helix-loop-helix-PASproteinMOP1;bHLHe78;ClassEbasichelix-loop-helixprotein78;HIF1A;HIF1alpha;HIF-1-alpha;HIF1A;HIF1Alpha;HIF1;HIF1-alpha;HIF1A;HIF1A_HUMAN;HypoxiainducIBLefactor1alpha;Hypoxiainduciblefactor1alphaisoformI.3;Hypoxiainduciblefactor1alphasubunit;Hypoxiainduciblefactor1alphasubunitbasichelixloophelixtranscriptionfactor;Hypoxiainduciblefactor1,alphasubunit(basichelixloophelixtranscriptionfactor);Hypoxiainduciblefactor1alpha;Hypoxia-induciblefactor1-alpha;Hypoxia-induciblefactor-1a;MemberofPASprotein1;MemberofPASsuperfamily1;MemberofthePASSuperfamily1;MOP1;MOP1;PASdomain-containingprotein8;PASD8;PASD8.

研究领域	肿瘤细胞生物神经生物学信号转导细胞凋亡
抗体来源	Mouse
克隆类型	Monoclonal
克隆号	1A7
交叉反应	Human,
产品应用	WB=1:500-1000 （石蜡切片需做抗原修复） notyettestedinotherapplications.optimaldilutions/concentrationsshouldbedeterminedbytheenduser.
分子量	92kDa
细胞定位	细胞核细胞浆
性状	LyophilizedorLiquid
浓度	1mg/ml
免疫原	RecombinanthumanHIF-1Alpha:
亚型	IgM
纯化方法	affinitypurifiedbyProteinAGL
储存液	0.01MTBS(pH7.4)with1%BSA,0.03%Proclin300and50%Glycerol.
保存条件	Storeat-20°Cforoneyear.Avoidrepeatedfreeze/thawcycles.Thelyophilizedantibodyisstableatroomtemperatureforatleastonemonthandforgreaterthanayearwhenkeptat-20°C.WhenreconstitutedinsterilepH7.40.01MPBSordiluentofantibodytheantibodyisstableforatleasttwoweeksat2-4°C.
PubMed	PubMed
产品介绍	background:Hypoxia-induciblefactor-1(HIF1)isatranscriptionfactorfoundinmammaliancellsculturedunderreducedoxygentensionthatplaysanessentialroleincellularandsystemichomeostaticresponsestohypoxia.HIF1isaheterodimercomposedofanalphasubunitandabetasubunit.Thebetasubunithasbeenidentifiedasthearylhydrocarbonreceptornucleartranslocator(ARNT).ThisgeneencodesthealphasubunitofHIF-1.Overexpressionofanaturalantisensetranscript(aHIF)ofthisgenehasbeenshowntobeassociatedwithnonpapillaryrenalcarcinomas.Twoalternativetranscriptsencodingdifferentisoformshavebeenidentified.Function:Functionsasamastertranscriptionalregulatoroftheadaptiveresponsetohypoxia.Underhypoxicconditionsactivatesthetranscriptionofover40genes,including,erythropoietin,glucosetransporters,glycolyticenzymes,vascularendothelialgrowthfactor,andothergeneswhoseproteinproductsincreaseoxygendeliveryorfacilitatemetabolicadaptationtohypoxia.Playsanessentialroleinembryonicvascularization,tumorangiogenesisandpathophysiologyofischemicdisease.BindstocoreDNAsequence5-[AG]CGTG-3withinthehypoxiaresponseelement(HRE)oftargetgenepromoters.ActivationrequiresrecruitmentoftranscriptionalcoactivatorssuchasCREBPBandEP300.Activityisenhancedbyinteractionwithboth,NCOA1orNCOA2.InteractionwithredoxregulatoryproteinAPEXseemstoactivateCTADandpotentiatesactivationbyNCOA1andCREBBP.Subunit:InteractswithCOPS5subunitofCOP9signalosomecomplex,leADIngtotheregulationofitsstABIlity.InteractswithTSGA10(Bysimilarity).EfficientDNAbindingrequiresheterodimerizationofanalphaandabeta/ARNTsubunit.BindstotheTAZ-type1domainsofCREBBPandEP300.InteractswithNCOA1,NCOA2,APEXandHSP90.InteractswithVHLwhichdocksHFA1totheE3ubiquitinligasecomplexforsubsequentdestruction.Interaction,viatheODDdomain,withthebetadomainofVHLL,protectsHIF1AfromdestructionbycompetingagainstthedestructivetargetinginitiatedbyVHL.SubcellularLocation:Cytoplasm.Nucleus.TissueSpecificity:Expressedinmosttissueswithhighestlevelsinkidneyandheart.Overexpressedinthemajorityofcommonhumancancersandtheirmetastases,duetothepresenceofintratumoralhypoxiaandasaresultofmutationsingenesencodingoncoproteinsandtumorsuppressors.Post-translationalmodifications:Innormoxia,ishydroxylatedonPro-402andPro-564intheoxygen-dependentdegradationdomain(ODD)byEGLN1/PHD1andEGLN2/PHD2.EGLN3/PHD3hasalsobeenshowntohydroxylatePro-564.ThehydroxylatedprolinespromoteinteractionwithVHL,initiatingrapidubiquitinationandsubsequentproteasomaldegradation.Underhypoxia,prolinehydroxylationisimpairedandubiquitinationisattenuated,resultinginstabilization.Innormoxia,ishydroxylatedonAsn-803byHIF1AN,thusabrogatinginteractionwithCREBBPandEP300andpreventingtranscriptionalactivation.S-nitrosylationofCys-800mayberesponsibleforincreasedrecruitmentofp300coactivatornecessaryfortranscriptionalactivityofHIF-1complex.AcetylationofLys-532byARD1increasesinteractionwithVHLandstimulatessubsequentproteasomaldegradation.RequiresphosphorylationforDNA-binding.Similarity:Contains1basichelix-loop-helix(bHLH)domain.Contains1PAC(PAS-associatedC-terminal)domain.Contains2PAS(PER-ARNT-SIM)domains.SWISS:Q16665GeneID:3091Databaselinks: EntrezGene:3091Human EntrezGene:15251Mouse Omim:603348Human SwissProt:Q16665Human SwissProt:Q61221Mouse Unigene:597216Human Unigene:3879Mouse Unigene:446610Mouse ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplications.

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