产品描述

RegularagaroseGradeCatalogNO.STA1200

Agarose

Storedryatroomtemperature

Descriptions:Size:Formulation:

100g

WhitePowder

Introduction:

Routineuseagaroseisidealforeverydayanalysisofnucleicacidsbygelelectrophoresisorblotting(NorthernorSouthern)andisalsosuitableforproteinapplicationssuchasOuchterlonyandrADIalimmunodiffusion(RID).

Data:

EEO(electroendosmosis)(-Mr)

0.15

Watercontent

≤10%

Sulfate(SO4)

≤0.15%

Gelstrength(1.0%gel)

≥1000/cm2

Meltingpoint(1.0%gel)

87.5±1.5℃

Gellingpoint(1.0%gel)

35.5±1.5℃

Foreignactivity

DNase,RNase,nonedetected

AnalysisNote:

Sulfatecontent-usedasanindicatorofpurity,sincesulfateisthemajorionicgrouppresent.

Gelstrength-theforcethatmustbeappliedtoageltocauseittofracture.

Gelpoint-thetemperatureatwhichanaqueousagarosesolutionformsagelasitcools.Agarosesolutionsexhibithysteresisintheliquid-to-geltransition-thatis,theirgelpointisnotthesameastheirmeltingtemperature.

Electroendosmosis(EEO)-amovementofliquidthroughthegel.Anionicgroupsinanagarosegelareaffixedtothematrixandcannotmove,butdissociablecountercationscanmigratetowardthecathodeinthematrix,givingrisetoEEO.Sinceelectrophoreticmovementofbiopolymersisusuallytowardtheanode,EEOcandisruptseparationsbecauseofinternalconvection.

PreparationofAgaroseGelsforDNAseparations:

WeighoutthedesiredamountofagaroseandplaceinanErlenmeyerflaskwithameasuredamountofelectrophoresisbuffer,e.g.foran0.8%gel,add0.8gmofagaroseand100mlofTBEBuffer(1X),toa200mlflask.Thelargerflaskinsuresagainsttheagaroseboilingover.Dissolvetheagaroseinaboilingwaterbathorinarevolving-platemicrowaveoven.Allthegrainsofagaroseshouldbedissolvedandthesolutionclear.Coolthesolutionto60°C(70°Cforconcentrations2%orabove)andpourimmediately.Allowthegeltosetforone-halfhourbeforeusing.Makesuretousethesameelectrophoresisbufferinthegelasfortherunningbuffer.