DIG寡核苷酸5′末端标记试剂盒DIGOligonucleotide5′-EndLabelingSet：Setfor10labelingreactions产品号产品规格厂商价格148086311480863001Set(10个标记反应)Roche5841DIGOligonucleotide5-EndLabelingSetApplicationThesetisusedfor5-endlabelingofoligonucleotideswithdigoxigenin.Theoligonucleotideisreactedinafinalsynthesisstepwithanaminolinkerphosphoramidite,creatinga5-terminalaminofunction.Aftercompletionoftheoligonucleotidesynthesis,thedigoxigeninmoietyisintroducedatthe5position.Ingeneral,1mgNHS-esterissufficientforthelabelingofapproximately300nmolofamino-substitutedoligonucleotide.Figure1:Typicalelutionprofileofadigoxigenin-labeledoligonucleotide.ProductDescriptionStability:Thecompoundsofthesetarestableinsolidformforatleastoneyearwhenstoredat15to25°C.Thephosphoramiditesolutionisstableforapproximatelytwoweeksatambienttemperaturewiththeexclusionofmoisture.ThesolutionoftheNHS-esterinethanolisstableonlyforashorttime.Therefore,theestersolutionshouldbeusedforseveralparallellabelings.Contents1.“Aminolinker”[N-Trifluoroacetamido-(3-oxa)-pentyl-N,N-diisopropyl-methyl]phosphoramidite,100mg2.Digoxigenin-NHS-ester,(Digoxigenin-3-0-methylcarbonyl-e-aminocaproicacid-N-hydroxy-succinimideester),5x1mg