• ImmunofluorescenccMicroscopyoftissueculturecells(MicroscopyandElectronicImagingLab)ThesemethodsarewrittenfordirectstainingoffilamentousactinwithbodipyFL-phallicidinandindirectimmunofluorescencestainingofmicrotubuleswithanti-tubulinantibodies.Theyeasilycanbemodifiedforconventionalimmunofluorescencestainingofanytissueculturedcellordirectstainingofactinfilaments
• Photomicrography(BowtellLab)
• StainingNucleicAcidswithPropidiumIodide(MolecularDynamicsInc)Propidiumiodideisamembrane-impermeantdyethatstainsbyintercalatingintonucleicacidmolecules.ItbindsbothDNAandRNA.Itisusefulinconfocalmicroscopystudiesofchromosometopography,DNAstructure,andmembraneintegrity.Itcanalsobeusedindouble-labelexperimentstodetectDNAandafluorescein-labeledantigensimultaneously.Thisprotocolcanbeusedtostaincellswithpropidiumiodidealone,simultaneouslywithanotherFluorochrome,orafterstainingwithanotherfluorochrome.
• ImmunfluoresenceonChamberSlides(BowtellLab)
• Mountingmediumforimmunofluorescencemicroscopy(HouseEarInstitute)Thismountingmediumiseasytoprepare,canbestoredcoldorfrozen,canbeusedwithanti-fadeagentsandwillpolymerizetogiveadry,semi-permanentmount.Ifforsomereasonthesampleisneededagain,immesinginwaterwilldissolvethemediumagain.

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