德国罗氏试剂，部分常用现货，订货两到三周，每周三订单
http://www.runwelltac.com

CellDeathDetectionELISA^PLU,细胞凋亡ELISA检测试剂盒
11774425001,1kitforupto96tests
Assaytime:
3-4hours
Negativecontrol:
Dependingoncellcultureconditions,eachexponentiallygrowingpermanentcellculturecontainsacertainamountofdeadcells(typicallyapproximately3-8%).Intheimmunoassay,theseinherentdeadcellsintheuntreatedsample(withoutacell-death-inducingagent)willcauseacertainabsorbancevalue(negativecontrol).
Positivecontrol:
ADNA-histonecomplexservesasapositivecontrol.
Samplematerial:
Cytoplasmicfractions(lysates)ofcelllines,cellsexvivo,cellculturesupernatants,andserumorplasma
Sensitivity:
Theexactdetectionlimitofdying/deadcellsinaparticularsamplestronglydependsonthekineticsofcelldeath,thecytotoxicagentused,andtheamountofaffectedcellsinthetotalcellpopulation.UsingU937/camptothecin(CAM)asacellularmodelsystemforcelldeath,theimmunoassayallowsthespecificdetectionofmono-andoligonucleosomesinthecytoplasmicfractionof125cellequivalents/well.
Specificity:
Anti-histonereactswiththehistonesH1,H2A,H2B,H3,andH4ofvariousspecies(e.g.,human,mouse,rat,hamster,cow,opossum,Xenopus).Anti-DNAbindstosingle-anddouble-strandedDNA.Therefore,theELISAallowsthedetectionofmono-andoligonucleosomesfromvariousspecies,andmaybeappliedtomeasureapoptoticcelldeathinmanydifferentcellsystems.