细胞转染

预优化DNA转染试剂BHK21

Description

GenJet™DNA InVitro TranfectionReagentfor BHK-21cellispre-optimizedfortransfectingBHK-21cells. BHK-21cellsisoneofthemostcommonlyusedcellsforvaccineproduction.Whenusedforthisapplication,usuallyacellstrainadaptedtogrowinsuspensionisused. Refertothefollowingoptimaltransfectionconditionsformaximaltransfectionefficiencyon BHK-21 cells.GenJet™reagent,1.0ml,issufficientfor300to600transfectionsin24wellplatesor150to300transfectionsin6wellplates.

SummaryofOptimalTransfectionConditions:ConfluenceonthedayoftransfectionCellcultureconditionsGenJet™(µl):DNA(µg)RatioDiluentforDNAandTransfectionReagentIncubationTimetoFormGenJet™/DNAComplexPresenceofSerum/AntibioticsduringTransfectionChangeMediumAfterTransfectionMaximalEfficiency TransfectionResults:ReporterGenePlasmidEfficiency(GFP%)

80%DMEMwith4.5g/Lglucose,10%FBS3:1Serum-freeDMEMwith4.5g/Lglucose15minutesatRTYesYes,5HoursAfterTransfection48hoursEGFPpEGFP-N3(CMVpromoter) 85%

StorageConditionStoreat4°C.Ifstoredproperly,theproductisstablefor12monthsorlonger

DataSheet.pdf

================  蚂蚁淘在线  ================

免责声明:本文仅代表作者个人观点,与本网无关。其创作性以及文中陈述文字和内容未经本站证实,对本文以及其中全部或者部分内容、文字的真实性、完整性、及时性本站不做任何保证或承诺,请读者仅作参考,并请自行核实相关内容

版权声明:未经蚂蚁淘在线授权不得转载、摘编或利用其他方式使用上述作品。已经经本网授权使用作品的,应该授权范围内使用,并注明“来源:蚂蚁淘在线”。违反上述声明者,本网将追究其相关法律责任。