Type

Recombinant

Description

BoneMorphogeneticProtein-2 HumanRecombinantproducedinE.Coliisahomodimeric,non-glycosylated,Polypeptidechaincontaining115 aminoacidsandhavingamolecularmassof26018 Dalton.TheBMP-2 ispurifiedbyproprietarychromatographictechniques.

AminoAcidSequence

ThesequenceofthefirstfiveN-terminalaminoacidswasdeterminedandwasfoundtobeMet-Gln-Ala-Lys-His.

Source

E.coli

Purity

Greaterthan95.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.

BiologicalActivity

1. TheED50asdeterminedbythecytolysisofMC3T3-E1cellsis<50 ng/ml.2. Whenimplantedinthemousethighbonemuscleandgrowfor14 days,Muscletissuewasharvestedandexaminedbiochemicallyforcalciumuptake.Ourunitdefinedas1 microgramcalciumabsorbed.Thespecificactivityismorethan1000 Units/mg.3. TheactivityofBMP-2 wastestedusingalkalinephosphatase,theactivityinmousemusclemyoblastC2C12celllinewasfoundtobe1×105U/mgprotein.4. Theectopicosteogenesiscapacityis1000mgfreshboneper1mgof BMP-2.

Formulation

BMP-2 waslyophilizedfromaconcentrated(1mg/ml)sterilesolutioncontaining20mMNaAc pH=4.

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

LyophilizedBoneMorphogeneticProtein-2 althoughstableatroomtemperaturefor3 weeks,shouldbestoreddesiccatedbelow –18°C.UponreconstitutionBMP2shouldbestoredat4°Cbetween2–7daysandforfutureusebelow –18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.

PhysicalAppearance

Sterilefilteredwhitelyophilized(freeze-dried)powder.