品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com

Agrisera/KC1 | potassium channel KAT3/AS09 515/

作者: 时间:2024-09-20 点击量:

product information
Background

KC1 is a regulatory K+ channel subunit that assembles with different inward-rectifying K+ channels to affect their activities. The protein is expressed in extremely low amounts, e.g. a few houndred per cell. Alternative names: AKT4, AtKC1, potassium channel TKC

Immunogen

KLH-conjugated peptide derived from Arabidopsis thaliana KC1 protein P92960, At4g32650

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized
Quantity 200 µg
Reconstitution For reconstitution add 100 µl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS09 516 | anti-AKT1 | potassium channel AKT1

collection of antibodies to membrane transport system

Plant protein extraction buffer

Secondary antibodies

Additional information

for detection images please, refer to the publication below

Antibody detects native and recombinant KC1.

application information
Recommended dilution

1: 50 with 125I (WB)

Expected | apparent MW

75.5 | 75 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity

Arabidopsis thaliana

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

In the work of Honsbein et al. 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning. ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems. In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell).

Selected references Honsbein et al. (2009). A tripartite SNARE-K+ channel complex mediates in channel-dependent K+ nutrition in Arabidopsis. The Plant Cell 21:2859-2877.

>>> 更多资讯详情请访问蚂蚁淘商城

Expression Systems商品列表
图片/货号 产品名/品牌 价格/货期 操作