| application information | ||
| Recommended dilution | 1: 50 with 125I (WB) | |
| Expected | apparent MW | 96.9 kDa | |
| Confirmed reactivity | Arabidopsis thaliana | |
| Predicted reactivity | Arabidopsis thaliana | |
| Not reactive in | no confirmed exceptions from predicted reactivity known in the moment | |
| Additional information | In the work of Honsbein et al. 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning. ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems. In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell). | |
| Selected references | Safiarian et al. (2015). Lost in traffic? The K+ channel of lily pollen, LilKT1, is detected at the endomembranes inside yeast cells, tobacco leaves and lily pollen. Front. Plant Sci. | doi: 10.3389/fpls.2015.00047. Honsbein et al. (2009). A tripartite SNARE-K+ channel complex mediates in channel-dependent K+ nutrition in Arabidopsis. The Plant Cell 21:2859-2877. |
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