Product Specification:

Alternative Name:	Heat shock protein 40
Clone:	2E1
Host:	Mouse
Isotype:	IgG2a
Immunogen:	Recombinant human Hsp40 (Hdj1).
UniProt ID:	P25685
GenBank ID:	D49547
Species reactivity:	Human, Mouse, Rat
Applications:	IP, WB
Recommended Dilutions/Conditions:	Immunoprecipitation (2-5µg/200µl from 5x106cells) Western Blot (1-2µg/ml, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Application Notes:	Detects a band of ~40kDa by Western blot.
Purity Detail:	Protein A affinity purified.
Formulation:	Liquid. In PBS, pH 7.2, containing 50% glycerol.
Shipping:	Shipped on Blue Ice
Long Term Storage:	-20°C
Scientific Background:	Hsp40/Hdj1 is a cytosolic co-chaperone belonging to the class DnaJ, named after its homolog in E. coli. Hsp40 functions in protein folding by binding nascent peptides and unfolded substrates and facilitating substrate interaction with Hsp70. Binding of Hsp40 to Hsp70 increases Hsp70 ATP hydrolysis and substrate binding, which is reversed by the action of nucleotide exchange factors (GrpE in E. coli). Repeated cycles of Hsp40/Hsp70 peptide binding and ATP hydrolysis prevent premature folding and aggregation, promoting protein maturation throughout the cell.

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