Product Specification:
| Alternative Name: | RELA |
| Host: | Rabbit |
| Immunogen: | Synthetic peptide corresponding to a portion of human p65 NF-κB. The sequence is completely conserved in mouse NF-κB p65. |
| UniProt ID: | Q04206 |
| GenBank ID: | M62399 |
| Species reactivity: | Human, Mouse, RatBovine, Dog, Guinea pig, Monkey, Porcine, Rabbit |
| Applications: | WB |
| Recommended Dilutions/Conditions: | Western Blot (1:1,000, colorimetric)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
| Application Notes: | Detects a band of ~65kDa by Western blot. |
| Purity Detail: | Protein A affinity purified. |
| Formulation: | Liquid. In PBS, pH 7.2, containing 0.09% sodium azide. |
| Handling: | Avoid freeze/thaw cycles. |
| Shipping: | Shipped on Blue Ice |
| Long Term Storage: | -20°C |
| Scientific Background: | Nuclear factor kappa-B (NFk) B) functions as a sequence specific transcriptional activator that binds to the intronic enhancer of kappa light chain gene in B lymphocytes. NF-kB is a heterodimer consisting of a 50 kDa DNA binding subunit (p50) and a 65 kDa transactivation subunit (p65/RelA) (ref. 3), both of which exhibit sequence homology to the protooncogene c-Rel. p50 has an isoform called p49/p52, and both proteins derive from the amino-terminal of precursor protein p105 and p100, respectively. The p50/p65 heterodimer remains in inactive form in the cytosol as a complex with its inhibitor, IkB. Upon stimulation of cells by a variety of stimuli, including lipopolysaccharide (LPS), pro-inflammatory cytokines like interleukin-1 and tumor necrosis factor, and viral infection, IkB becomes phosphorylated and degraded by the proteosome. The active NF-kB heterodimer translocates into the nucleus and induces gene expression. |
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