Product Description

C3/C5-Depleted Serum is normal human serum in which C3 and C5 have been removed by immunoaffinity chromatography. The product is tested for the absence of C3 and C5 activity by testing classical and alternative pathway function and for the absence of C3 and C5 proteins by double immunodiffusion. The C3/C5-depleted serum is certified to exhibit less than 5% classical pathway and alternative pathway activities. Functional classical and alternative pathways can be reconstituted by addition of purified C3 protein (1.3 mg/mL) and C5 protein (75 ug/mL) indicating that all other complement components necessary for classical and alternative pathway activation are present. After reconstitution with C3 (1.3 mg C3/mL) and C5 (0.75 mg/mL), the C3/C5-Depleted Serum is certified to possess a functional classical and alternative pathway for complement activation (Morgan, B.P. (2000); Dodds, A.W. and Sim, R.B. (1997)). Although a functional lectin pathway should also be reconstituted by the addition of similar concentrations of C3 and C5, the function of this pathway is not tested.

(For full product description see link...)

Complement Technology的GVB o是一种基本缓冲液，可用于制备用于补体测定的其他传统缓冲液。可以将Ca ++和Mg ++添加到GVB o中，以制成用于经典途径和凝集素途径测定的GVB ++。可以将MgEGTA添加到GVB o中以进行替代途径测定。EDTA可被添加到GVB Ò准备GVBE抑制补体活化。GVB ø也用于血清和其它测定组分的稀释在许多补体测定中特别是在旁路途径测定（摩根，BP（2000;多兹，AW和Sim，RB（1997））。