Product Description

Natural human C5ais prepared from human C5 protein cleaved into C5a and C5b by human C5 convertase.The C5a is converted to C5a desArg by proteolytic removal of the C-terminal arginine.The primary carboxypeptidase responsible for Arg removal is serum carboxypeptidase N, but there are several different carboxypepticases in serum.C5a desArg is a naturally glycosylated polypeptide containing 73 amino acids with a molecular weight of approx. 10,250 daltons.It contains 25% carbohydrate attached to a single Asn residue at position 64. This carbohydrate is of variable structure leading to a broad distribution of MW upon analysis by mass spectroscopy.C5a is the most potent anaplylatoxin (compared to C3a and C4a).C5a desArg is produced when C5a is “inactivated” by removal of its C-terminal arginine amino acid.This cleavage occurs by the action of the plasma enzyme carboxypeptidase N.This inactivation is rapid and most C5a is converted to C5a desArg within minutes of its formation.“Inactivated” C5a still possesses approx. 1% of its anaphylatoxic and chemotatic activities, but its stimulatory activity is only reduced 10-fold.Thus, C5a desArg retains considerable biological activity even though it is frequently called inactivated C5a.Its biological properties include being weakly chemotactic for neutrophils (PMN), causing smooth muscle contraction, increasing vascular permeability, causing histamine and TNF-alpharelease, and causing lysosomal degranulation of immune cells.C5a and C5a desArg act through the C5a Receptor (C5aR, CD88, a G-protein coupled receptor) on PMN, monocytes, alveolar macrophages, and mast cells.A second receptor of unknown function (C5L2, gpr77) has been identified.Due to the widespread expression of C5a receptors and the results from C5aR KO mice it is believed that C5a and its receptors have many non-immunolgical functions in organ development, CNS development, neurodegeneration, tissue regeneration and hematopoiesis (Monk, P.N. et al. (2007)).

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Complement Technology的GVB o是一种基本缓冲液，可用于制备用于补体测定的其他传统缓冲液。可以将Ca ++和Mg ++添加到GVB o中，以制成用于经典途径和凝集素途径测定的GVB ++。可以将MgEGTA添加到GVB o中以进行替代途径测定。EDTA可被添加到GVB Ò准备GVBE抑制补体活化。GVB ø也用于血清和其它测定组分的稀释在许多补体测定中特别是在旁路途径测定（摩根，BP（2000;多兹，AW和Sim，RB（1997））。