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Abnova/FFPE FISH PreTreatment Kit 1/1kit/KA2375

价格
面议
货号:KA2375
浏览量:127
品牌:Abnova
服务
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商品描述
  • Specification
  • Product Description:
  • Pre-treatment is an essential step for effective FISH on Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections. This kit contains key reagents used to pretreat FFPE tissue sections from dewaxing with xylene through proteolytic digestion to helps permeabilization of cell membranes to facilitate penetration of fluorescence labeled probes. After proteolytic digestion, proceed the subsequent steps with the FISH assay protocol.
  • Supplied Product:
  • Kit content:1. 1x Paraffin Pretreatment Solution, 500 mL2. Protease (Pepsin), 0.5 mL x 10 vials
  • Storage Instruction:
  • After arrival, please store the Paraffin Pretreatment Solution at room temperature (RT), and store the Protease (Pepsin) at -20°C.
  • Protocol:
  • PDF DownloadProtocol Download
  • MSDS:
  • PDF DownloadDownload
  • Regulation Status:
  • For research use only (RUO)
  • Datasheet:
  • PDF DownloadDownload
  • Publication Reference
  • 1.
  • Deficiency of PTEN and CDKN2A Tumor-Suppressor Genes in Conventional and Chondroid Chordomas: Molecular Characteristics and Clinical Relevance.Chenlong Yang, Jianjun Sun, Lei Yong, Chen Liang, Tie Liu, Yulun Xu, Jun Yang, Xiaoguang Liu.Onco Targets Ther. 2020 May 25;13:4649-4663. doi: 10.2147/OTT.S252990. eCollection 2020.
  • 2.
  • Clinical significance of PD-1/PD-Ls gene amplification and overexpression in patients with hepatocellular carcinoma.Ma LJ, Feng FL, Dong LQ, Zhang Z, Duan M, Liu LZ, Shi JY, Yang LX, Wang ZC, Zhang S, Ding ZB, Ke AW, Cao Y, Zhang XM, Zhou J, Fan J, Wang XY, Gao Q.Theranostics. 2018 Nov 10;8(20):5690-5702. doi: 10.7150/thno.28742. eCollection 2018.
  • 3.
  • FGF19 genetic amplification as a potential therapeutic target in lung squamous cell carcinomas.Zhang X, Kong M, Zhang Z, Xu S, Yan F, Wei L, Zhou J.Thorac Cancer. 2017 Sep 14. [Epub ahead of print]
  • 4.
  • Investigation of Molecular Alterations of AKT-3 in Triple Negative Breast Cancer.O"Hurley G, Daly E, O"Grady A, Cummins R, Quinn C, Flanagan L, Pierce A, Fan Y, Lynn MA, Rafferty M, Fitzgerald D, Pont幯 F, Duffy MJ, Jirstr闣 K, Kay EW, Gallagher WM.Histopathology. 2013 Oct 19. doi: 10.1111/his.12313.
  • Applications
  • Fluorescent In Situ Hybridization (Cell)
  • PDF DownloadProtocol Download
  • Application Image
  • Fluorescent In Situ Hybridization (Cell)
Abnova的Abnova Diagnostics F蛋白表达系统基于小麦胚芽的真核翻译设备。小麦胚在浓缩干燥状态下储存所有翻译成分,一旦发芽就准备好进行蛋白质合成。常规的小麦胚芽提取物包含RNA N-糖苷酶三丁酸酯和其他翻译抑制剂,例如硫蛋白,核糖核酸酶,脱氧核糖核酸酶和蛋白酶。这些抑制剂来自胚乳。大量洗涤小麦胚芽以消除胚乳污染物已导致提取物具有高度的稳定性和活性。通过将具有5'cap和多(RSA)尾巴的mRNA与该提取物与专有脂质体结合使用,进行体外翻译反应产生足够量的膜蛋白,该膜蛋白被脂质体捕获,导致正确的构象和生物学功能必不可少的折叠。该系统相对于普遍使用的蛋白质表达平台(例如大肠杆菌,昆虫细胞和哺乳动物细胞)具有明显优势,这些平台都是体内系统,并且在细胞膜的约束下导致产量和稳定性低。此外,体外小麦胚芽系统适于自动化以用于高通量小分子以及生物学筛选和表征。