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Abnova/12(S)-HETE ELISA Kit/1kit/KA0306

价格
面议
货号:KA0306
浏览量:127
品牌:Abnova
服务
全国联保
正品保证
正规发票
签订合同
商品描述
  • Specification
  • Product Description:
  • 12(S)-HETE ELISA Kit is a competitive immunoassay for the quantitative determination of 12(S)-HETE in biological fluids.
  • Calibration Range:
  • 195 to 50000 pg/mL
  • Limit of Detection:
  • 146 pg/mL
  • Quality Control Testing:
  • Standard curveQC Testing of KA0306The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
  • Storage Instruction:
  • The standard and conjugate must be stored at -20°C; others store at 4°C.Avoid repeated freezing and thawing.
  • Protocol:
  • PDF DownloadProtocol Download
  • MSDS:
  • PDF DownloadDownload
  • Suitable Sample:
  • Cell Culture Supernatant, Plasma
  • Sample Volume:
  • 100 uL
  • Label:
  • AP-conjugate
  • Detection Method:
  • Colorimetric
  • Intra-Assay:
  • 10.26%
  • Inter-Assay:
  • 11.30%
  • Spiking Recovery:
  • Cell Culture Supernatant: 94.00%, Plasma (Heparin): 104.00%, Plasma (EDTA): 97.00%
  • Regulation Status:
  • For research use only (RUO)
  • Datasheet:
  • PDF DownloadDownload
  • Publication Reference
  • 1.
  • 2,3",4,5"-Tetramethoxystilbene prevents deoxycorticosterone-salt-induced hypertension: contribution of cytochrome P-450 1B1.Sahan-Firat S, Jennings BL, Yaghini FA, Song CY, Estes AM, Fang XR, Farjana N, Khan AI, Malik KU.Am J Physiol Heart Circ Physiol. 2010 Dec;299(6):H1891-901. Epub 2010 Sep 17.
  • Applications
  • Quantification
  • Application Image
  • Quantification
Abnova的Abnova Diagnostics F蛋白表达系统基于小麦胚芽的真核翻译设备。小麦胚在浓缩干燥状态下储存所有翻译成分,一旦发芽就准备好进行蛋白质合成。常规的小麦胚芽提取物包含RNA N-糖苷酶三丁酸酯和其他翻译抑制剂,例如硫蛋白,核糖核酸酶,脱氧核糖核酸酶和蛋白酶。这些抑制剂来自胚乳。大量洗涤小麦胚芽以消除胚乳污染物已导致提取物具有高度的稳定性和活性。通过将具有5'cap和多(RSA)尾巴的mRNA与该提取物与专有脂质体结合使用,进行体外翻译反应产生足够量的膜蛋白,该膜蛋白被脂质体捕获,导致正确的构象和生物学功能必不可少的折叠。该系统相对于普遍使用的蛋白质表达平台(例如大肠杆菌,昆虫细胞和哺乳动物细胞)具有明显优势,这些平台都是体内系统,并且在细胞膜的约束下导致产量和稳定性低。此外,体外小麦胚芽系统适于自动化以用于高通量小分子以及生物学筛选和表征。