品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
- Specification
- Product Description:
- This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the ACTN1 protein, and the other against the CTNNA1 protein for use in in situ Proximity Ligation Assay. See Publication Reference below.
- Reactivity:
- Human
- Quality Control Testing:
- Protein protein interaction immunofluorescence result.
Representative image of Proximity Ligation Assay of protein-protein interactions between ACTN1 and CTNNA1. HeLa cells were stained with anti-ACTN1 rabbit purified polyclonal antibody 1:1200 and anti-CTNNA1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
- Supplied Product:
- Antibody pair set content: 1. ACTN1 rabbit purified polyclonal antibody (100 ug) 2. CTNNA1 mouse monoclonal antibody (40 ug) *Reagents are sufficient for at least 30-50 assays using recommended protocols.
- Storage Instruction:
- Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
- MSDS:
Download
- Datasheet:
- Download
- Publication Reference
- 1.
- An analysis of protein-protein interactions in cross-talk pathways reveals CRKL as a novel prognostic marker in hepatocellular carcinoma.Liu CH, Chen TC, Chau GY, Jan YH, Chen CH, Hsu CN, Lin KT, Juang YL, Lu PJ, Cheng HC, Chen MH, Chang CF, Ting YS, Kao CY, Hsiao M, Huang CY. Mol Cell Proteomics. 2013 Feb 8. [Epub ahead of print]
- Applications
- In situ Proximity Ligation Assay (Cell)
- Application Image
- In situ Proximity Ligation Assay (Cell)
- ACTN1
- CTNNA1
- Gene Information
- Entrez GeneID:
- 87
- Gene Name:
- ACTN1
- Gene Alias:
- FLJ40884
- Gene Description:
- actinin, alpha 1
- Omim ID:
- 102575
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
- Other Designations:
- F-actin cross-linking protein,actinin 1 smooth muscle,alpha-actinin 1
- Gene Information
- Entrez GeneID:
- 1495
- Gene Name:
- CTNNA1
- Gene Alias:
- CAP102,FLJ36832
- Gene Description:
- catenin (cadherin-associated protein), alpha 1, 102kDa
- Omim ID:
- 116805
- Gene Ontology:
- Hyperlink
- Other Designations:
- alpha-E-catenin,alpha-catenin,alphaE-catenin,cadherin-associated protein,102kDa,catenin (cadherin-associated protein), alpha 1 (102kD),catenin, alpha 1
- Interactome 1
- Interactome 2
