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苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
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0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
- Specification
- Product Description:
- Labeled FISH probes for identification of gene translocation using Fluorescent In Situ Hybridization Technique. (Technology)
- Form:
- Liquid
- Quality Control Testing:
- Representative images of normal human cell (lymphocyte) stain with the dual color FISH probe. The left image is chromosomes at metaphase, and the right image is an interphase nucleus.
- Supplied Product:
- DAPI Counterstain (1500 ng/mL ) 125 uL for each 100 uL FISH Probe
- Storage Instruction:
- Store at 4°C in the dark.
- Note:
- Hybridization position of the probes on the chromosome.
- Probe 1:Size:Fluorophore:Location:
- IGHApproximately 1550kbFITC14q32
- Probe 2:Size:Fluorophore:Location:
- BCL2Approximately 820kbTexas Red18q21.3
- Origin:
- Human
- Source:
- Genomic DNA
- Notice:
- We strongly recommend the customer to use FFPE FISH PreTreatment Kit 1 (Catalog #: KA2375 or KA2691) for the pretreatment of Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.
- Regulation Status:
- For research use only (RUO)
- Datasheet:
Download
- Applications
- Fluorescent In Situ Hybridization (Cell)
- Protocol Download
- Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)
enlarge this image- human cholangiocarcinoma (FFPE) stained with 1IGH/BCL2,DY FISH Probe . human cholangiocarcinoma showed no 1IGH/BCL2 translocation
- Protocol Download
- Application Image
- Fluorescent In Situ Hybridization (Cell)
- Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)
- enlarge
- BCL2
- IGH
- Gene Information
- Entrez GeneID:
- 3492
- Gene Name:
- IGH
- Gene Alias:
- IGH,IGH.1@,IGHDY1,MGC72071,MGC88774
- Gene Description:
- immunoglobulin heavy locus
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. This region represents the germline organization of the heavy chain locus. The locus includes V (variable), D (diversity), J (joining), and C (constant) segments. During B cell development, a recombination event at the DNA level joins a single D segment with a J segment; this partially rearranged D-J gene is then joined to a V segment. The rearranged V-D-J is then transcribed with the IGHM constant region; this transcript encodes a mu heavy chain. Later in development B cells generate V-D-J-Cmu-Cdelta pre-messenger RNA, which is alternatively spliced to encode either a mu or a delta heavy chain. Mature B cells in the lymph nodes undergo switch recombination, so that the V-D-J gene is brought in proximity to one of the IGHG, IGHA, or IGHE genes and each cell expresses either the gamma, alpha, or epsilon heavy chain. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase, and by somatic hypermutation, which occurs during B cell maturation in the spleen and lymph nodes. Several V, D, J, and C segments are known to be incapable of encoding a protein and are considered pseudogenes. [provided by RefSeq
- Other Designations:
- -
- Gene Information
- Entrez GeneID:
- 596
- Gene Name:
- BCL2
- Gene Alias:
- Bcl-2
- Gene Description:
- B-cell CLL/lymphoma 2
- Omim ID:
- 151430
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq
- Other Designations:
- B-cell lymphoma protein 2,OTTHUMP00000163680
