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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
- Specification
- Product Description:
- mutaFISH™ EGFR T790M T790wt RNA Probes is designed to detect human EGFR T790M gene mutation on single strand RNA in cells using padlock probe and in situ rolling-circle amplification technology.
- Supplied Product:
- Content:1. RT EGFR T790 Primer2. mutaFISH™ EGFR T790M RNA Probe3. mutaFISH™ EGFR T790wt RNA Probe4. Detection Probe-Aqua 4315. Detection Probe-Texas Red X
- Storage Instruction:
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
- Note:
- We recommend mutaFISH™ RNA Accessory Kit (Catalog #: KA4915) which provides necessary reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
- Protocol:
Protocol Download
- Technology:
- mutaFISH™ (mutation-specific Fluorescence In Situ Hybridization)
- Comparison:
- FISH Probes vs mutaFISH™ Probes
- Fluorophore:
- Aqua 431 (Excitation Peak (nm): 431; Emission Peak (nm): 480)Texas Red X (Excitation Peak (nm): 595; Emission Peak613)
- Probe Position:
- Regulatory Status:
- For research use only (RUO)
- Datasheet:
- Download
- Video:
- Applications
- mutation specific, Fluorescence In Situ Hybridization (Cells)
- mutaFISH™ staining was performed in situ in human H1975 cells captured by CytoQuest™ CR. EGFR T790M point mutation was detected via orange signal (Cy3), and EGFR T790wt was detected via red signal (Cy5).
- Application Image
- mutation specific, Fluorescence In Situ Hybridization (Cells)
- enlarge
- Gene Information
- Entrez GeneID:
- 1956
- Gene Name:
- EGFR
- Gene Alias:
- ERBB,ERBB1,HER1,PIG61,mENA
- Gene Description:
- epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
- Omim ID:
- 131550, 211980
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
- Other Designations:
- avian erythroblastic leukemia viral (v-erb-b) oncogene homolog,cell growth inhibiting protein 40,cell proliferation-inducing protein 61,epidermal growth factor receptor
- Interactome
- Gene Pathway
- Adherens junction
- Bladder cancer
- Calcium signaling pathway
- Colorectal cancer
- Cytokine-cytokine receptor interaction
- Dorso-ventral axis formation
- Endocytosis
- Endometrial cancer
- Epithelial cell signaling in Helicobacter pylori infection
- ErbB signaling pathway
- Focal adhesion
- Gap junction
- Glioma
- GnRH signaling pathway
- MAPK signaling pathway
- Melanoma
- Non-small cell lung cancer
- Pancreatic cancer
- Pathways in cancer
- Prostate cancer
- Regulation of actin cytoskeleton
- Related Disease
- Adenocarcinoma
- Adenocarcinoma, Bronchiolo-Alveolar
- Adenocarcinoma, Mucinous
- Adenocarcinoma, Papillary
- Anus Neoplasms
- Asthma
- Asthma
- Astrocytoma
- Atherosclerosis
- Barrett Esophagus
- Bile Duct Neoplasms
- Biliary Tract Neoplasms
- Bipolar Disorder
- Brain Neoplasms
- Breast cancer
- Breast Neoplasms
- Bronchial Hyperreactivity
- Carcinoma
- Carcinoma, Adenosquamous
