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商品描述
Myelin Basic Protein (Bovine), Biotin
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Product Description | The myelin basic protein (MBP) is isolated from bovine brain. Biotinylated MBP could enhance the efficiency of immobilization of the substrate on an avidin-coated microplate and the substrate would be readily accessible for kinase activity through a large molecule avidin as a spacer. | ![]() SDS-PAGE profile of biotinylated MBP | |
Species | Bovine (brain from Canadian source) | ||
Formulation | 2 mg/mL in 0.1M MOP, pH 7 | ||
Isolation | Ion Exchange Chromatography | ||
Purification | G15 Sephadex Gel Filtration | ||
Biotinylation | N-hydroxysuccinimidobiotin | ||
Biotin/MBP | 5:1 | ||
Applications | Substrate for kinase assay | ||
Scientific Description | Myelin consists of 80% lipid fat and 20% protein. It is an electrically insulating phospholipid layer that surrounds the axons of many neurons. Myelin basic protein (MBP) is a protein responsible for myelination of the neurons of the central nervous system. | ||
Storage & Stability | The product is stable at 4°C for 12 months. For extended storage, aliquot content and store product at -20°C. Expiration date for extended storage is one year from date of shipping if stored properly. | ||
Product Specific References | 1. J. Biol. Chem. 1971. 246(18): 5770-5784. |