The RealHelix^TM Direct qPCR Kit [Probe] is designed for a probe-based qPCR amplification directly from animal tissues, plant tissues, and various clinical samples including whole blood, serum, urine, hairand swab collections without any DNA purification processes. The 2x Direct qPCR Premix [probe] in this kit contains antibody-inhibited Taq DNA polymerase, dNTPs, MgCI₂, stabilizer, and unique buffer system to resist various PCR inhibitors of tissue samples.

Figure 1. Real-time PCR using RealHelix Direct qPCR Kit [Probe] from various human samples. The lysates from each sample (whole blood, buccal swab, or urine) were prepared following the protocol of this kit. Each 1 μl of lysate and 10 ng human genomic DNA(positive control) were analyzed using a human beta-globin gene-specific primer set and a probe labeled with FAM on a Real-Time PCR instrument, Bio-Rad CFX96. NTC, no template control. Each reaction was duplicated.

 Application 

• Direct and quantitative real-time PCR 

Contents 

• 2x Direct qPCR Premix [Probe]
• 3x P-solution
• 10x Dilution Buffer

Quality control assay data

RealHelix^TM ​Direct qPCR Kit [Probe] is evaluated by real-time PCRs with target-specific primers and hydrolysis probes using whole blood or plant leaf according to the protocol supplied in this kit.