- Overview
- Data/Specifications
- Literature/Support
- How To Use
- Related Products
Overview
Recognizes δ unsaturated disaccharide of unsulfated chondroitin generated by chondroitinase ABC digestion.
Data/Specifications
Immunogen: Bovine nasal cartilage aggrecan that had been deglycosylated with chondroitinase ABCdigestion; i.e. BNC (ABC) Core (see Couchman et al 1984 and Caterson et al 1985).
Clone: 1B5
Concentration: 0.1 mg/mL
Isotype: IgG1K
Host: Mouse
Purity: Protein A
Specificity: This antibody recognizes zero sulphated Chondroitin Sulphate stub neoepitope generated by chondroitinase ABC treatment.
Form: Clear liquid; PBS, no preservatives
Storage: -20° C
Literature/Support
Chondroitin Sulphate Neoepitope Antibody (Insert)
A.J. Hayes et al. (2008) Methods 45:10–21
Couchman JR, Caterson B, Christner JB, Baker JR (1984) Mapping by monoclonal antibody detection of glycosaminoglycans in connective tissues. Nature 307:650–652
Caterson B, Christner JE, Baker JR, Couchman JR (1985) Production and characterization of monoclonal antibodies directed against connective tissue proteoglycans. Fed Proc 44:386–393
Anthony J. Hayes, Amanda Hall, Liesbeth Brown, Ross Tubo, and Bruce Caterson (2007) Macromolecular Organization and In Vitro Growth Characteristics of Scaffold-free Neocartilage GraftsJournal of Histochemistry & Cytochemistry Volume 55(8): 853–866
How To Use
Applications:
- Western Blot: use at dilution of 1/100.
- Immunohistochemistry: Formalin or paraformaldehyde-fixed paraffin embedded sections, alcohol-fixed frozen sections or un-fixed snap-frozen sections.
Technical Notes:
Samples are usually deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10ug S-GAG of non-deglycosylated aggrecan for optimal epitope recognition in SDS-PAGE and immunohistochemistry.
