YANG Bin;LIU Xin-ming;Miyazawa Teruo;WANG Jin-ye;Shanghai JiaoTong University;Institute of Organic Chemistry, CAS;Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University;Objective: To investigate physical stability, drug delivery and cytocompatiblity of a cholesterol derivative(CTBBA)combined liposome. Methods: CTBBA was combined with liposome; and zeta potential was analyzed in buffers with different/gradient pH. Size distribution and phosphorus content analysis were carried out to investigate the physical stability of liposome. The effect of CTBBA on drug release profile in vitro was studied. The cytotoxicity of CTBBA or CTBBA-liposome was measured. Finally the intracellular drug delivery was evaluated by flow cytometry analysis and fluorescence microscope. Results: The zeta potential of CTBBA was positive, which improved the physical stability of liposome during the two year storage period. As a cholesterol derivative,CTBBA-liposome could inhibit the drug leakage in vitro. As a positive charged carrier, CTBBA showed a lower cytotoxicity. Flow cytometry analysis could not exactly reveal the intracellular distribution of DOX-liposome, and pretreatment of fixation changed the distribution of DOX-liposome. Conclusion: Positively charged CTBBA-liposome could improve the physical stability with low cytotoxicity. Flow cytometry analysis could not exactly reveal the intracellular distribution of DOX-liposome. 【Fund】： 国家自然科学基金项目(30870635 31070868);;国家基础研究计划973项目(2005CB724306);;国家国际科技合作计划(2012DFA30270) 【CateGory Index】： R730.5 Download(CAJ format) Download(PDF format) CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format. Lv Jingning;Cui Huaijiang;Department of Chemistry and Chemical Engineering,Jining University;;Recent Developments in Affinity Polymer Latex Microspheres[J];Chemical Industry Times;2013-12 SONG Yong-yu;SUN Yu-qi;HU Hai-yang;QIAO Ming-xi;ZHAO Xiu-li;CHEN Da-wei;College of Pharmaceutical Sciences,Zhejiang Chinese Medical University;School of Pharmacy,Shenyang Pharmaceutical University;;Preparation and evaluation of doxorubicin loaded polyamide-amine dendrimer locked in liposome[J];Journal of Shenyang Pharmaceutical University;2013-09 ZHU Yan1,ZHU Xiong2,ZHOU Jinpei1(1.Department of Medicinal Chemistry,China Pharmaceutical University,Nanjing 210009,China;2.Medicinal and Chemical Institute,China Pharmaceutical University,Nanjing 210009,China);Recent Progress in Research on Aza-anthrones with Antitumor Activity[J];Progress in Pharmaceutical Sciences;2013-07 Hongwei ZHAO;Jincheng LI;Department of Anesthesiology, Tianjin Medical University Cancer Institute and Hospital; National Clinical Center of Cancer; Key Laboratory of Cancer Prevention and Therapy,Tianjin;;Research progress on the effect of anesthesia on anthracycline cardiotoxicity[J];Chinese Journal of Clinical Oncology;2013-22 Shu-Guang Zheng;Hui-Xiong Xu;Hang-Rong Chen;Department of Medical Ultrasound, Shanghai Tenth People’s Hospital, Tenth People’s Hospital of Tongji University;State Key Laboratory of High Performance Ceramic and Superfine Microstructures, Shanghai Institute of Ceramic Chinese Academy of Science;;Nano/microparticles and ultrasound contrast agents[J];世界放射学杂志(电子版)(英文版);2013-12 LI Fangyuan;JIANG Yongli;CHENG Ying;Department of Pharmaceutics,School of Pharmacy,the Fourth Military Medical University;Department of Pharmacy,Tangdu Hospital,the Fourth Military Medical University;;Progress in the study of poly(lactide-co-glycolide)nanoparticles for anticancer agents delivery[J];Northwest Pharmaceutical Journal;2013-06 Qianyu Zhang,Jie Tang,Ling Fu,Rui Ran,Yayuan Liu,Mingqing Yuan,Qin He (Key Laboratory of Drug Targeting and Drug Delivery Systems,West China School of Pharmacy,Sichuan University.No.17,Block 3,Southern Renmin Road,Chengdu 610041,P.R.China);A pH-responsive a-helical cell penetrating peptide-mediated liposomal delivery system[A];[C];2013 ZHANG Li-jun,SHI Hui-qin,YUAN Xiao-yan,YU Hai-tao,ZHAO Jun,PENG Shuang-qing (Research and Evaluation Center for Toxicology,Institute of Disease Prevention and Control, Academy of Military Medical Sciences,Beijing 100071,China);Cardiotoxicity of doxorubicin on embryonic zebrafish[A];[C];2013

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