ProductDescription
Bortezomibisafirst-in-classdipeptideboronicacid-based,water-solubleproteasomeinhibitor.Asasingleagent,bortezomibwasfoundtohaveconsistentantitumoractivityinbothchemosensitiveandchemoresistantmultiplemyelomacellsatanIC50of10-20ng/mL.[1]BortezomibovercomestheresistancetoapoptosisinmultiplemyelomacellsthatisinducedbyIL-6.[2]Additionally,bortezomibpreventsTNF-a-induced,NF-kB-dependentupregulationofIL-6andreducescelladhesion;proliferationofremainingadherentmultiplemyelomacellswasalsoinhibitedbybortezomib.[1]
InMMcelllinesU266,IM-9,andHsSultan,bortezomibinhibitedatIC50concentrationsof3,6,and20nM,respectively.[2]CellgrowthofDox40,MR20,andLR5MMcellswascompletelyinhibitedbybortezomibat100nMIC50.
BortezomibsuppressesgrowthandinducesapoptosisinBcr/Abl-positivecellssensitiveandresistanttoIM.Interestingly,sequentialcombinationofbortezomibfollowedbyimatinibresultedinasynergisticpro-apoptoticeffectinimatinib-resistantcells;simultaneousexposureofbortezomibandimatinibwasantagoNISTic.[3]
Technicalinformation:
| ChemicalFormula: | C19H25BN4O4 | |
| CAS#: | 179324-69-7 | |
| MolecularWeight: | 384.24 | |
| Purity: | >98% | |
| Appearance: | LightYellowCrystalline | |
| ChemicalName: | [(1R)-3-methyl-1-({(2S)-3-phenyl-2-[(pyrazin-2-ylcarbonyl)amino]propanoyl}amino)butyl]boronicacid | |
| Solubility: | Upto100mMinDMSO | |
| Synonyms: | PS-341,PS341,Bortezomib,Velcade,LDP-341 |
ShippingCondition:Theproductisshippedinaglassvialatambienttemperature.
Storagecondition:Forlongershelflife,storesolidpowderat4oCdesiccated,orstoreDMSOsolutionat-20oC.
Reference:
| 1. | Richardsonetal.,CancerControl,2003,10(6),361-369. |
| 2. | Hideshimaetal.,TheproteasomeinhibitorPS-341inhibitsgrowth,inducesapoptosis,andovercomesdrugresistanceinhumanmultiplemyelomacells.CancerRes.2001,61,3071-3076.PubmedID:1306489 |
| 3. | Gattoetal.,TheproteasomeinhibitorPS-341inhibitsgrowthandinducesapoptosisinBcr/Abl-positivecelllinessensitiveandresistanttoimatinibmesylate.Haematologica,2003,88(8),853-863.PubmedID:12935973 |
Cellagen的ChamQ SYBR qPCR预混液（无ROX）| Q321产品描述ChamQSYBR®qPCR预混液（Vazyme，＃Q321）由香槟Taq DNA聚合酶（Vazyme，＃P122）通过抗体修饰的热启动激活技术保护，是专为基于SYBR Green I的定量PCR（qPCR）设计的。ChamQSYBR®qPCR Master Mix的优化缓冲液系统中的独特因素（即促进特异性的Exactor）大大提高了其灵敏度和特异性。该混合物以2倍反应浓度制备，可直接用于鲁棒性低模板qPCR，具有高灵敏度，特异性和可靠性。优点特异性+敏感性的最佳组合。独特的抗体修饰的热启动taq，具有独特的特异性促进因子。Mg2 +和染料的最佳浓度。
