ClonExpress UItra One Step Cloning kit (Vazyme, #C115) is a novel cloning kit, applicable for homologous recombination with 1 - 5 fragments. The Kit is independent of DNA ligase, significantly reducing the self-ligated colonies. The enhanced Exnase and highly optimized buffer included in 2x ClonExpress Mix significantly improve the recombination efficiency and the tolerance to impurities. pCE-Zero vector is compatible with most PCR products, enabling the specific PCR products to be used directly for recombination without any treatments, which significantly simplify the procedure.

ClonExpress One Step Cloning is simple, fast, and highly efficient cloning technology which enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly, the vector is linearized at the cloning site. A small sequence overlapped with each end of the cloning site is added onto the insert through PCR. The insert and the linearized vector, with overlapped sequences of 15 bp - 20 bp on both 5’- and 3’-end, respectively, are mixed in an appropriate ratio and incubated with recombinase Exnase at 50°C for 5 - 15 min.Product Manual (pdf)

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Advantages

• Easy: one-tube super mix.
• Fast: cloning within 5 - 15 min.
• Efficient cloning of fragments of 50 bp-10 kb, with positive clone rate > 95%. Ligase-independent.
• Directional cloning at any site on any vector, no limits in restriction enzyme choice.
• Suitable for cloning of both single and multiple DNA fragments.

When order, Cold Shipping option have to be selected, or your order may be delayed.

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Selected Product Citations

1. Xing Y, et al. SLERT Regulates DDX21 Rings Associated with Pol I Transcription. Cell, 2017, 169(4):664-78. Pubmed ID:28475895
2. Wu N, et al. TBX6 null variants and a common hypomorphic allele in congenital scoliosis. N Engl J Med, 2015, 4793:341-50. Pubmed ID:25564734
3. Ge J, et al. Architecture of the mammalian mechanosensitive Piezo1 channel. . Pubmed ID: Nature, 2015, 527(7576):64-9. Pubmed ID:26390154
4. Zhang XO, et al. Complementary Sequence-Mediated Exon Circularization. Cell, 2014, 159(1):134-47. Pubmed ID:25242744
5. Zong Y, et al. Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A. Nature Biotechnology, 2018. Pubmed ID:30272679
6. Li X, Wang Y, Liu Y, et al. Base editing with a Cpf1-cytidine deaminase fusion. Nature Biotechnology, 2018, 36(4):324-7. Pubmed ID:29553573

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Mechanism

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Storage

All components should be Store at -20°C. Avoid repeated freeze and thaw.

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Components

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Related Products

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4. ClonExpress MultiS One Step Cloning Kit (Vazyme, #C113)
5. Mut Express II Fast Mutagenesis Kit V2 (Vazyme, #C214)
6. Mut ExpressMultiS Fast Mutagenesis Kit V2 (Vazyme, #C215)

Cellagen的ChamQ SYBR qPCR预混液（无ROX）| Q321产品描述ChamQSYBR®qPCR预混液（Vazyme，＃Q321）由香槟Taq DNA聚合酶（Vazyme，＃P122）通过抗体修饰的热启动激活技术保护，是专为基于SYBR Green I的定量PCR（qPCR）设计的。ChamQSYBR®qPCR Master Mix的优化缓冲液系统中的独特因素（即促进特异性的Exactor）大大提高了其灵敏度和特异性。该混合物以2倍反应浓度制备，可直接用于鲁棒性低模板qPCR，具有高灵敏度，特异性和可靠性。优点特异性+敏感性的最佳组合。独特的抗体修饰的热启动taq，具有独特的特异性促进因子。Mg2 +和染料的最佳浓度。