ClonExpress II One Step Cloning (Vazyme, #C112) is a simple, fast, and high efficient cloning technology which is based on a homologous recombination technology. It enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly, the vector is linearized at the cloning site. A small sequence overlapped with each end of the cloning site is added onto the insert through PCR. The insert and the linearized vector, with overlapped sequences of 15 bp-20 bp on both 5’- and 3’-end, respectively, are mixed and incubated with Exnase II at 37°C for 30 min. The cloning products can then be directly transformed to competent cells with a true positive rate > 95%. This kit contains Exnase II and an optimized buffer with a unique recombinant enhancer which significantly improves the recombination efficiency. In addition, Exnase II is compatible with the reaction systems of restriction enzyme digestion and PCR, which means that both the digestion products of vector and the PCR products of insert can be directly used for recombination without purification, significantly simplifying the procedures.Product Manual (pdf)

---

Advantages

• Easy, fast, and efficient.
• Directional cloning at any site on any vector.
• No need to consider the restriction enzyme cutting sites carried on inserts.
• Ligase-independent, true positive rate > 95%.
• Efficient cloning of fragments of 50 bp-10 kb.
• Linearized vector and PCR products can be used directly without purification.

When order, Cold Shipping option have to be selected, or your order may be delayed.

---

Selected Product Citations

1. Xing Y, et al. SLERT Regulates DDX21 Rings Associated with Pol I Transcription. Cell, 2017, 169(4):664-78. Pubmed ID:28475895
2. Wu N, et al. TBX6 null variants and a common hypomorphic allele in congenital scoliosis. N Engl J Med, 2015, 4793:341-50. Pubmed ID:25564734
3. Ge J, et al. Architecture of the mammalian mechanosensitive Piezo1 channel. . Pubmed ID: Nature, 2015, 527(7576):64-9. Pubmed ID:26390154
4. Zhang XO, et al. Complementary Sequence-Mediated Exon Circularization. Cell, 2014, 159(1):134-47. Pubmed ID:25242744
5. Zong Y, et al. Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A. Nature Biotechnology, 2018. Pubmed ID:30272679
6. Li X, Wang Y, Liu Y, et al. Base editing with a Cpf1-cytidine deaminase fusion. Nature Biotechnology, 2018, 36(4):324-7. Pubmed ID:29553573

---

Mechanism

---

Storage

All components should be Store at -20°C.

---

Components

---

Related Products

1. 2 × Phanta Max Master Mix (Vazyme #P515)
2. Phanta Max Super-Fidelity DNA Polymerase (Vazyme #P505)
3. ClonExpress Ultra One Step Cloning Kit (Vazyme, #C115)
4. ClonExpress MultiS One Step Cloning Kit (Vazyme, #C113)
5. Mut Express II Fast Mutagenesis Kit V2 (Vazyme, #C214)
6. Mut ExpressMultiS Fast Mutagenesis Kit V2 (Vazyme, #C215)

Cellagen的ChamQ SYBR qPCR预混液（无ROX）| Q321产品描述ChamQSYBR®qPCR预混液（Vazyme，＃Q321）由香槟Taq DNA聚合酶（Vazyme，＃P122）通过抗体修饰的热启动激活技术保护，是专为基于SYBR Green I的定量PCR（qPCR）设计的。ChamQSYBR®qPCR Master Mix的优化缓冲液系统中的独特因素（即促进特异性的Exactor）大大提高了其灵敏度和特异性。该混合物以2倍反应浓度制备，可直接用于鲁棒性低模板qPCR，具有高灵敏度，特异性和可靠性。优点特异性+敏感性的最佳组合。独特的抗体修饰的热启动taq，具有独特的特异性促进因子。Mg2 +和染料的最佳浓度。